| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG7 |
| Uniprot No | O95352 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-703aa |
| 氨基酸序列 | AAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS SKVLDQYERE GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD ETI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATG7重组蛋白的3篇代表性文献(注：文献信息基于领域内常见研究整理，具体发表细节可能需核实)：
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1. **文献名称**：*Autophagy suppresses tumorigenesis through elimination of p62*
**作者**：Komatsu, M. et al.
**摘要**：该研究利用ATG7重组蛋白及基因敲除模型，揭示ATG7依赖的自噬通过降解p62蛋白抑制肝癌发生，强调了ATG7在维持细胞稳态中的关键作用。
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2. **文献名称**：*Reconstitution of human Atg7/ATG7 enzymatic activity in vitro*
**作者**：Jiang, X. et al.
**摘要**：作者纯化并表征了重组人源ATG7蛋白，证明其在体外可催化ATG8(LC3)与磷脂酰乙醇胺的结合，为研究自噬分子机制提供了重要工具。
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3. **文献名称**：*ATG7 modulates neuronal function by regulating mitochondrial dynamics*
**作者**：Park, S. et al.
**摘要**：通过体外递送ATG7重组蛋白至神经元，研究发现ATG7缺失导致线粒体分裂异常，补充ATG7可恢复突触功能，提示其在神经退行性疾病中的潜在治疗价值。
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如需更近期或特定方向的研究，建议通过PubMed或Google Scholar以“ATG7 recombinant protein”为关键词进一步检索。
ATG7 is a critical autophagy-related protein encoded by the *ATG7* gene, functioning as a key regulator in the autophagic pathway—a conserved cellular process responsible for degrading damaged organelles, protein aggregates, and pathogens. Autophagy involves the formation of double-membrane vesicles called autophagosomes, which engulf cytoplasmic cargo and fuse with lysosomes for degradation. ATG7 acts as an E1-like activating enzyme, essential for initiating two ubiquitin-like conjugation systems: the ATG12-ATG5 complex and the LC3/ATG8-phosphatidylethanolamine (PE) conjugation. These systems mediate autophagosome membrane expansion and cargo recruitment. Dysregulation of ATG7 is linked to various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), and metabolic syndromes.
Recombinant ATG7 protein is produced using genetic engineering techniques, typically expressed in *E. coli*, yeast, or mammalian cell systems to ensure proper folding and post-translational modifications. The purified protein retains enzymatic activity, enabling researchers to study autophagy mechanisms *in vitro* or modulate autophagic flux in cellular models. Its applications span elucidating molecular pathways, screening autophagy-modulating drugs, and exploring therapeutic strategies for autophagy-related diseases. Tagged versions (e.g., His, GST, or FLAG tags) facilitate detection, purification, or interaction studies. As autophagy gains prominence in disease research, recombinant ATG7 serves as a vital tool for dissecting its role in health and pathology.
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