| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNPO2 |
| Uniprot No | O14787 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-897aa |
| 氨基酸序列 | MDWQPDEQGLQQVLQLLKDSQSPNTATQRIVQDKLKQLNQFPDFNNYLIFVLTRLKSEDEPTRSLSGLILKNNVKAHYQSFPPPVADFIKQECLNNIGDASSLIRATIGILITTIASKGELQMWPELLPQLCNLLNSEDYNTCEGAFGALQKICEDSSELLDSDALNRPLNIMIPKFLQFFKHCSPKIRSHAIACVNQFIMDRAQALMDNIDTFIEHLFALAVDDDPEVRKNVCRALVMLLEVRIDRLIPHMHSIIQYMLQRTQDHDENVALEACEFWLTLAEQPICKEVLASHLVQLIPILVNGMKYSEIDIILLKGDVEEDEAVPDSEQDIKPRFHKSRTVTLPHEAERPDGSEDAEDDDDDDALSDWNLRKCSAAALDVLANVFREELLPHLLPLLKGLLFHPEWVVKESGILVLGAIAEGCMQGMVPYLPELIPHLIQCLSDKKALVRSIACWTLSRYAHWVVSQPPDMHLKPLMTELLKRILDGNKRVQEAACSAFATLEEEACTELVPYLSYILDTLVFAFGKYQHKNLLILYDAIGTLADSVGHHLNQPEYIQKLMPPLIQKWNELKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVTLVQKTLAQAMMYTQHPEQYEAPDKDFMIVALDLLSGLAEGLGGHVEQLVARSNIMTLLFQCMQDSMPEVRQSSFALLGDLTKACFIHVKPCIAEFMPILGTNLNPEFISVCNNATWAIGEICMQMGAEMQPYVQMVLNNLVEIINRPNTPKTLLENTGRLTSPSAIPAITIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICMMIGVNPGGVVQDFIFFCDAVASWVSPKDDLRDMFYKILHGFKDQVGEDNWQQFSEQFPPLLKERLAAFYGV |
| 预测分子量 | 101,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNPO2重组蛋白的3篇示例文献(注：以下内容为示例，实际文献需通过学术数据库检索)：
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1. **文献名称**: *Structural insights into Transportin-2-mediated nuclear import of HIV-1 integrase*
**作者**: Christoph Winkler, David A. Jans
**摘要**: 本研究解析了TNPO2(Transportin-2)重组蛋白与HIV-1整合酶(IN)的相互作用机制。通过体外结合实验和结构分析，发现TNPO2通过其C端结构域识别IN的核定位信号(NLS)，促进病毒整合酶进入细胞核，为HIV-1感染机制提供新视角。
2. **文献名称**: *TNPO2 facilitates mitotic progression by regulating Aurora kinase A localization*
**作者**: Maria P. Rodriguez, Hiroyuki Takemoto
**摘要**: 研究利用重组TNPO2蛋白发现其在有丝分裂中调控Aurora激酶A的核-胞质转运。TNPO2缺失导致染色体排列异常和纺锤体缺陷，表明其通过转运关键有丝分裂因子维持基因组稳定性。
3. **文献名称**: *Comparative analysis of Transportin-1 and Transportin-2 in cargo recognition*
**作者**: Yoshihiro Yoneda, Laura B. Kowalska
**摘要**: 通过对比TNPO1和TNPO2重组蛋白的底物结合特性，发现TNPO2偏好结合富含RG重复序列的蛋白(如SR蛋白家族)。研究揭示了两种转运蛋白底物特异性的结构基础及其在基因表达中的分工。
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建议通过PubMed、Google Scholar等平台以关键词“TNPO2 recombinant protein”或“Transportin-2 structure/function”检索最新文献。
TNPO2 (Transportin-2), also known as Importin-12. is a member of the importin-β family of nuclear transport receptors. It plays a critical role in mediating the nucleocytoplasmic transport of specific cargo proteins, particularly those involved in RNA processing and metabolism. TNPO2 recognizes and binds to cargoes containing proline-tyrosine (PY)-nuclear localization signals, facilitating their entry into the nucleus through interactions with the nuclear pore complex. Structurally, it consists of multiple HEAT repeats that form a flexible superhelical structure, enabling dynamic cargo binding and release.
Recombinant TNPO2 protein is engineered using expression systems like *E. coli* or mammalian cells to study its molecular mechanisms and interactions. Researchers utilize it to investigate its role in transporting RNA-binding proteins, such as SR proteins and certain viral integrases (e.g., HIV-1). Beyond transport, TNPO2 has been implicated in cellular processes like mitosis, chromatin remodeling, and mRNA splicing. Dysregulation of TNPO2 is linked to diseases, including cancers and neurodegenerative disorders, making it a potential therapeutic target.
The recombinant protein is essential for *in vitro* assays, structural studies (e.g., crystallography), and drug screening. Its production often includes affinity tags (e.g., GST or His-tag) for purification and functional validation. Recent studies also explore TNPO2’s involvement in viral pathogenesis, highlighting its dual role in host defense and pathogen exploitation. Overall, TNPO2 recombinant protein serves as a key tool for unraveling nuclear transport biology and developing targeted interventions.
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