| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFR2 |
| Uniprot No | Q9UP52 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-801aa |
| 氨基酸序列 | MERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPEPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRGSCQACGDSVLVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAALTQDIRAALSRQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPAQPISADIASRLLRKLKGPVAPQEWQGSLLGSPYHLGPGPRLRLVVNNHRTSTPINNIFGCIEGRSEPDHYVVIGAQRDAWGPGAAKSAVGTAILLELVRTFSSMVSNGFRPRRSLLFISWDGGDFGSVGSTEWLEGYLSVLHLKAVVYVSLDNAVLGDDKFHAKTSPLLTSLIESVLKQVDSPNHSGQTLYEQVVFTNPSWDAEVIRPLPMDSSAYSFTAFVGVPAVEFSFMEDDQAYPFLHTKEDTYENLHKVLQGRLPAVAQAVAQLAGQLLIRLSHDRLLPLDFGRYGDVVLRHIGNLNEFSGDLKARGLTLQWVYSARGDYIRAAEKLRQEIYSSEERDERLTRMYNVRIMRVEFYFLSQYVSPADSPFRHIFMGRGDHTLGALLDHLRLLRSNSSGTPGATSSTGFQESRFRRQLALLTWTLQGAANALSGDVWNIDNNF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TFR2重组蛋白的模拟参考文献示例(建议通过PubMed或Google Scholar核实真实文献)：
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1. **文献名称**：*"Expression and purification of recombinant TFR2 for functional studies in iron metabolism"*
**作者**：Zhang Y, et al.
**摘要**：研究报道了在昆虫细胞表达系统中高效表达人源TFR2重组蛋白的方法，并通过表面等离子体共振技术验证其与转铁蛋白的结合动力学，揭示了TFR2在肝铁调节中的潜在作用。
2. **文献名称**：*"Crystal structure of the TFR2-transferrin complex reveals insights into hereditary hemochromatosis"*
**作者**：Wang X, et al.
**摘要**：通过X射线晶体学解析TFR2重组蛋白与转铁蛋白的复合物结构，发现特定结构域突变会破坏结合界面，为遗传性血色素沉着症的病理机制提供结构依据。
3. **文献名称**：*"Functional analysis of TFR2 mutations using recombinant protein models"*
**作者**：Martinez R, et al.
**摘要**：利用哺乳动物细胞表达的重组TFR2蛋白，评估临床突变体(如Y250C)对铁调素(hepcidin)信号通路的影响，表明部分突变导致铁敏感性的丧失。
4. **文献名称**：*"Development of a TFR2-based biosensor for iron status monitoring"*
**作者**：Chen L, et al.
**摘要**：研究构建了基于重组TFR2蛋白的荧光生物传感器，可实时检测血清转铁蛋白饱和度，为铁代谢疾病诊断提供新工具。
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**注意**：以上为模拟生成的参考文献，实际文献需通过学术数据库检索，推荐使用关键词“TFR2 recombinant protein”“transferrin receptor 2 expression”或结合具体研究领域(如结构、突变、诊断应用)进行精准查询。
**Background of TFR2 Recombinant Protein**
Transferrin receptor 2 (TFR2) is a transmembrane protein primarily involved in regulating systemic iron homeostasis. Unlike its homolog TFR1. which is ubiquitously expressed and critical for cellular iron uptake via transferrin-bound iron, TFR2 is predominantly expressed in hepatocytes and erythroid precursors. It plays a key role in sensing circulating iron levels and modulating the production of hepcidin, the master iron-regulatory hormone synthesized in the liver. TFR2 interacts with hemochromatosis protein (HFE) and other components of the iron-sensing pathway to adjust hepcidin levels, thereby controlling dietary iron absorption and systemic iron distribution.
Mutations in the *TFR2* gene are linked to hereditary hemochromatosis type 3. a disorder characterized by iron overload and organ damage. This association underscores TFR2's importance in maintaining iron balance. Recombinant TFR2 protein, produced through genetic engineering in systems like mammalian or insect cell cultures, retains the functional domains required for studying its interactions and mechanisms. Researchers use it to investigate iron metabolism disorders, develop therapeutic strategies, and create diagnostic tools for iron-related diseases.
Structurally, recombinant TFR2 includes extracellular, transmembrane, and cytoplasmic domains, enabling studies on its ligand-binding properties (e.g., interaction with transferrin or HFE) and signaling pathways. Recent studies also explore its potential role in cancer, as aberrant TFR2 expression has been observed in hepatocellular carcinoma. By providing a purified and bioactive form of the protein, TFR2 recombinant tools advance our understanding of iron biology and related pathologies, bridging gaps between basic research and clinical applications.
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