| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFDP1 |
| Uniprot No | Q14186 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-410aa |
| 氨基酸序列 | AKDAGLIEA NGELKVFIDQ NLSPGKGVVS LVAVHPSTVN PLGKQLLPKT FGQSNVNIAQ QVVIGTPQRP AASNTLVVGS PHTPSTHFAS QNQPSDSSPW SAGKRNRKGE KNGKGLRHFS MKVCEKVQRK GTTSYNEVAD ELVAEFSAAD NHILPNESAY DQKNIRRRVY DALNVLMAMN IISKEKKEIK WIGLPTNSAQ ECQNLEVERQ RRLERIKQKQ SQLQELILQQ IAFKNLVQRN RHAEQQASRP PPPNSVIHLP FIIVNTSKKT VIDCSISNDK FEYLFNFDNT FEIHDDIEVL KRMGMACGLE SGSCSAEDLK MARSLVPKAL EPYVTEMAQG TVGGVFITTA GSTSNGTRFS ASDLTNGADG MLATSSNGSQ YSGSRVETPV SYVGEDDEED DDFNENDEDD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TFDP1重组蛋白的示例参考文献(注：以下内容为模拟虚构，实际引用时请核实真实文献)：
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1. **标题**: *"Expression and Functional Analysis of Recombinant TFDP1 in E2F-Mediated Transcriptional Regulation"*
**作者**: Smith J, et al. (2020)
**摘要**: 该研究报道了人源TFDP1重组蛋白在大肠杆菌中的高效表达及纯化方法，并通过体外结合实验证实其与E2F家族成员的相互作用，揭示了TFDP1-E2F复合物对特定基因启动子的调控机制。
2. **标题**: *"Structural Insights into TFDP1 Dimerization and DNA Binding Using Crystallized Recombinant Protein"*
**作者**: Zhang Y, et al. (2021)
**摘要**: 通过X射线晶体学解析了重组TFDP1蛋白的三维结构，发现其N端结构域对二聚化至关重要，并鉴定了与DNA结合相关的关键氨基酸残基，为靶向设计调控TFDP1功能的分子奠定基础。
3. **标题**: *"Recombinant TFDP1 as a Tool for Screening Anticancer Agents Targeting Cell Cycle Progression"*
**作者**: Lee S, et al. (2019)
**摘要**: 研究开发了基于重组TFDP1蛋白的高通量筛选平台，用于鉴定抑制TFDP1-E2F相互作用的化合物，并验证了部分候选分子在抑制肺癌细胞增殖中的潜在效果。
4. **标题**: *"TFDP1 Knockdown and Recombinant Protein Rescue Experiments Reveal Its Role in Cellular Senescence"*
**作者**: Garcia R, et al. (2018)
**摘要**: 利用siRNA敲低TFDP1后，通过添加重组TFDP1蛋白成功恢复了细胞衰老表型，证明其在调控p53依赖性衰老通路中的必要性，并探讨了其翻译后修饰对功能的影响。
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(注：以上文献信息为示例性质，实际研究中请通过学术数据库如PubMed、Web of Science检索真实文献。)
**Background of TFDP1 Recombinant Protein**
TFDP1 (Transcription Factor Dp-1) is a critical regulatory protein involved in cell cycle progression and transcriptional regulation. It belongs to the DP family of transcription factors and functions primarily by forming heterodimers with E2F proteins (E2F1-6). These E2F/DP complexes bind to specific DNA sequences, activating or repressing the expression of genes essential for DNA replication, cell cycle entry, and apoptosis. TFDP1 enhances the DNA-binding affinity and stability of E2Fs, playing a pivotal role in transitioning cells from the G1 to S phase.
The recombinant TFDP1 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems* to ensure proper folding and post-translational modifications. It is purified using affinity tags (e.g., His-tag, GST-tag) and validated via SDS-PAGE, Western blot, or functional assays. Recombinant TFDP1 retains the ability to dimerize with E2Fs and bind DNA, making it a valuable tool for studying cell cycle mechanisms, protein-DNA interactions, and transcriptional regulation *in vitro*.
Dysregulation of TFDP1 is linked to cancers, as overexpression can drive uncontrolled proliferation by hyperactivating E2F-target genes. Consequently, recombinant TFDP1 is utilized in cancer research to dissect oncogenic pathways and screen therapeutic agents targeting the E2F/DP axis. It also aids in structural studies to map dimerization domains or DNA-binding regions, providing insights for drug design.
Overall, TFDP1 recombinant protein serves as a vital reagent for elucidating the molecular basis of cell cycle control and developing strategies to combat diseases associated with its dysfunction.
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