| 纯度 | >90%SDS-PAGE. |
| 种属 | E. coli |
| 靶点 | TKL2 |
| Uniprot No | P33315 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681aa |
| 氨基酸序列 | MAQFSDIDKLAVSTLRLLSVDQVESAQSGHPGAPLGLAPVAHVIFKQLRCNPNNEHWINRDRFVLSNGHSCALLYSMLHLLGYDYSIEDLRQFRQVNSRTPGHPEFHSAGVEITSGPLGQGISNAVGMAIAQANFAATYNEDGFPISDSYTFAIVGDGCLQEGVSSETSSLAGHLQLGNLITFYDSNSISIDGKTSYSFDEDVLKRYEAYGWEVMEVDKGDDDMESISSALEKAKLSKDKPTIIKVTTTIGFGSLQQGTAGVHGSALKADDVKQLKKRWGFDPNKSFVVPQEVYDYYKKTVVEPGQKLNEEWDRMFEEYKTKFPEKGKELQRRLNGELPEGWEKHLPKFTPDDDALATRKTSQQVLTNMVQVLPELIGGSADLTPSNLTRWEGAVDFQPPITQLGNYAGRYIRYGVREHGMGAIMNGISAFGANYKPYGGTFLNFVSYAAGAVRLAALSGNPVIWVATHDSIGLGEDGPTHQPIETLAHLRAIPNMHVWRPADGNETSAAYYSAIKSGRTPSVVALSRQNLPQLEHSSFEKALKGGYVIHDVENPDIILVSTGSEVSISIDAAKKLYDTKKIKARVVSLPDFYTFDRQSEEYRFSVLPDGVPIMSFEVLATSSWGKYAHQSFGLDEFGRSGKGPEIYKLFDFTADGVASRAEKTINYYKGKQLLSPMGRAF |
| 预测分子量 | 75 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TKL2重组蛋白的模拟参考文献示例(注：内容为假设性描述，仅供参考)：
1. **文献名称**：Expression and Functional Characterization of Recombinant TKL2 Kinase in Insect Cells
**作者**：Smith A, et al.
**摘要**：本研究利用杆状病毒表达系统成功表达并纯化了TKL2重组蛋白，证实其具有ATP结合活性和底物磷酸化能力，为激酶抑制剂筛选提供了工具。
2. **文献名称**：Structural Insights into TKL2 Recombinant Protein by Cryo-EM
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析了重组TKL2蛋白的高分辨率结构，揭示了其激酶结构域中关键活性位点的构象变化，为靶向药物开发奠定基础。
3. **文献名称**：TKL2 Recombinant Protein Enhances Wound Healing in Diabetic Mouse Models
**作者**：Wang X, et al.
**摘要**：实验表明，外源性重组TKL2蛋白通过激活ERK信号通路促进成纤维细胞迁移，显著加速糖尿病小鼠的皮肤创面修复。
4. **文献名称**：Optimization of TKL2 Recombinant Protein Production in E. coli for Industrial Applications
**作者**：Kim S, et al.
**摘要**：通过优化密码子使用和诱导条件，在大肠杆菌中实现了TKL2重组蛋白的高效可溶性表达，为规模化生产提供了可行方案。
**注意**：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索关键词“TKL2 recombinant protein”获取。
TKL2 (Tyrosine Kinase-Like 2) is a recombinant protein derived from the human TKL2 gene, which belongs to the receptor tyrosine kinase (RTK) superfamily. This protein plays a regulatory role in cellular signaling pathways, particularly those involved in cell proliferation, differentiation, and apoptosis. Structurally, TKL2 contains conserved kinase domains and extracellular ligand-binding regions, enabling its participation in phosphorylation-dependent signal transduction. Dysregulation of TKL2 has been implicated in pathological conditions, including cancers and inflammatory disorders, making it a potential therapeutic target.
The production of recombinant TKL2 typically involves cloning its coding sequence into expression vectors (e.g., bacterial, insect, or mammalian systems) to enable large-scale, high-purity yields. Affinity tags like His or GST are often fused to facilitate purification and detection. Recombinant TKL2 serves as a critical tool for in vitro studies, allowing researchers to analyze its enzymatic activity, substrate interactions, and response to inhibitors. It also aids in structural studies (e.g., X-ray crystallography) to elucidate mechanisms of kinase activation or drug binding.
Recent research focuses on TKL2’s role in modulating immune responses and tumorigenesis. For instance, its overexpression in certain cancers correlates with poor prognosis, suggesting its utility as a biomarker or a target for kinase inhibitors. Additionally, TKL2 recombinant protein is employed in drug screening platforms to identify small molecules that selectively inhibit its activity, potentially paving the way for novel therapies. Despite progress, challenges remain in understanding its tissue-specific functions and cross-talk with other signaling pathways, necessitating further investigation.
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