| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TKL4 |
| Uniprot No | A0A1Y3DN75 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性关于TKL4重组蛋白的参考文献范例(非真实文献，仅供格式参考)：
1. **文献名称**: "Expression and Functional Analysis of Recombinant TKL4 Protein in Cancer Cell Lines"
**作者**: Li, X.; Chen, Y.; Wang, H.
**摘要**: 本研究通过大肠杆菌系统表达了重组TKL4蛋白，验证其酪氨酸激酶活性，并发现其在抑制乳腺癌细胞增殖中具有潜在作用。
2. **文献名称**: "Structural Characterization of TKL4 Kinase Domain Using Recombinant Protein Technology"
**作者**: Smith, J.; Brown, K.
**摘要**: 利用昆虫细胞表达系统纯化TKL4激酶结构域重组蛋白，通过X射线晶体学解析其三维结构，为靶向药物设计提供依据。
3. **文献名称**: "TKL4 Recombinant Protein Enhances Neuronal Differentiation in vitro"
**作者**: Gupta, R.; Kim, S.
**摘要**: 研究证明，HEK293细胞表达的重组TKL4蛋白可通过激活MAPK信号通路促进神经干细胞向神经元分化。
4. **文献名称**: "Optimization of TKL4 Recombinant Protein Production in Pichia pastoris"
**作者**: Zhang, L.; Zhou, M.
**摘要**: 通过优化毕赤酵母表达系统的培养条件，实现TKL4重组蛋白的高效分泌表达，产量较原核系统提升5倍。
注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。
**Background of TKL4 Recombinant Protein**
TKL4 (Tyrosine Kinase-Like 4) is a recombinant protein engineered to mimic the structure and function of its native counterpart, a member of the tyrosine kinase-like family. This family shares structural similarities with receptor tyrosine kinases (RTKs) but lacks canonical kinase activity in certain members, suggesting diverse roles in cellular signaling. TKL4 is typically produced using recombinant DNA technology, often expressed in *E. coli* or mammalian systems, and purified to retain functional domains critical for interaction studies.
Biologically, TKL4 is implicated in modulating intracellular signaling pathways, potentially influencing cell proliferation, differentiation, and apoptosis. While its exact physiological role remains under investigation, studies suggest associations with developmental processes and disease pathways, including cancer. For instance, dysregulation of TKL4-related signaling may contribute to tumorigenesis by altering downstream effectors like MAPK or Wnt pathways.
The recombinant TKL4 protein serves as a vital tool for *in vitro* and *cell-based* assays to dissect its molecular mechanisms. Researchers utilize it to study protein-protein interactions, substrate phosphorylation, and pathway crosstalk. Additionally, it aids in drug discovery, enabling high-throughput screening for inhibitors or activators targeting TKL4-associated networks.
Despite progress, challenges persist in fully elucidating TKL4's kinase activity (or lack thereof) and its context-dependent roles. Its recombinant form, often tagged with fluorescent or affinity markers, facilitates structural studies (e.g., crystallography) to resolve these ambiguities. Overall, TKL4 recombinant protein remains a focal point in unraveling the complexity of pseudokinase biology and its therapeutic potential.
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