| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TKL8 |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TKL8重组蛋白的虚构参考文献示例，格式符合学术规范：
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1. **文献名称**: "Structural characterization and functional analysis of recombinant TKL8 protein in cancer cell signaling"
**作者**: Zhang, Y., et al.
**摘要**: 本研究通过大肠杆菌表达系统成功纯化TKL8重组蛋白，并利用X射线晶体学解析其三维结构。实验表明TKL8通过调控Wnt/β-catenin信号通路促进乳腺癌细胞迁移，为靶向治疗提供了潜在靶点。
2. **文献名称**: "High-yield production of bioactive TKL8 recombinant protein in insect cells using baculovirus expression"
**作者**: Müller, F., et al.
**摘要**: 开发了一种基于杆状病毒-昆虫细胞系统的高效TKL8重组蛋白生产策略，优化后的蛋白糖基化修饰显著增强了其体外促血管生成活性，适用于药物筛选及功能研究。
3. **文献名称**: "TKL8 recombinant protein as a novel biomarker for early-stage colorectal cancer detection"
**作者**: Tanaka, K., et al.
**摘要**: 通过ELISA检测发现血清中TKL8重组蛋白水平在结直肠癌患者中显著升高(p<0.001)，其诊断灵敏度达82%，表明其作为非侵入性生物标志物的临床应用潜力。
4. **文献名称**: "Antiviral activity of TKL8 recombinant protein against influenza A virus through JAK-STAT pathway modulation"
**作者**: Gupta, R., et al.
**摘要**: 首次报道TKL8重组蛋白通过激活JAK-STAT通路诱导干扰素产生，在体外实验中抑制甲型流感病毒复制(IC50=15nM)，为抗病毒药物开发提供了新方向。
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注：以上文献为模拟示例，实际研究中请通过专业数据库检索真实文献。如需特定领域文献建议，请提供更详细的研究背景。
**Background of TKL8 Recombinant Protein**
TKL8 (Tyrosine Kinase-Like 8) recombinant protein is a engineered biomolecule derived from the TKL family, a subgroup of the protein kinase superfamily. Protein kinases play pivotal roles in cellular signaling by catalyzing the transfer of phosphate groups to target proteins, thereby regulating diverse biological processes, including cell proliferation, differentiation, and apoptosis. The TKL family, distinct from classical tyrosine or serine/threonine kinases, exhibits unique structural features and substrate specificities, making it an area of growing interest in both basic and applied research.
TKL8. as a member of this family, is implicated in evolutionary conserved signaling pathways. Its recombinant form is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to ensure high purity and activity for experimental applications. The recombinant protein retains the kinase domain critical for its enzymatic function, often fused with tags (e.g., His-tag or GST) to facilitate purification and detection.
Studies on TKL8 are limited but suggest its potential involvement in stress responses, immune regulation, and developmental processes. For instance, homologs in model organisms (e.g., *Arabidopsis* or *Drosophila*) have been linked to signaling cascades affecting growth and environmental adaptation. In humans, dysregulation of kinase activity is frequently associated with diseases like cancer, inflammation, and neurodegeneration, positioning TKL8 as a candidate for exploring novel therapeutic targets.
Current research focuses on elucidating TKL8's substrate profiles, activation mechanisms, and interaction networks. Recombinant TKL8 serves as a vital tool for *in vitro* assays, structural studies (e.g., X-ray crystallography), and inhibitor screening. Challenges remain in fully characterizing its physiological roles, partly due to functional redundancy within the kinase family. Nonetheless, advancements in recombinant protein technology and CRISPR-based functional genomics are accelerating discoveries, highlighting TKL8's relevance in both cellular homeostasis and disease contexts.
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