Recombinant Human TKL8 protein

**Background of TKL8 Recombinant Protein**

TKL8 (Tyrosine Kinase-Like 8) recombinant protein is a engineered biomolecule derived from the TKL family, a subgroup of the protein kinase superfamily. Protein kinases play pivotal roles in cellular signaling by catalyzing the transfer of phosphate groups to target proteins, thereby regulating diverse biological processes, including cell proliferation, differentiation, and apoptosis. The TKL family, distinct from classical tyrosine or serine/threonine kinases, exhibits unique structural features and substrate specificities, making it an area of growing interest in both basic and applied research.

TKL8. as a member of this family, is implicated in evolutionary conserved signaling pathways. Its recombinant form is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to ensure high purity and activity for experimental applications. The recombinant protein retains the kinase domain critical for its enzymatic function, often fused with tags (e.g., His-tag or GST) to facilitate purification and detection.

Studies on TKL8 are limited but suggest its potential involvement in stress responses, immune regulation, and developmental processes. For instance, homologs in model organisms (e.g., *Arabidopsis* or *Drosophila*) have been linked to signaling cascades affecting growth and environmental adaptation. In humans, dysregulation of kinase activity is frequently associated with diseases like cancer, inflammation, and neurodegeneration, positioning TKL8 as a candidate for exploring novel therapeutic targets.

Current research focuses on elucidating TKL8's substrate profiles, activation mechanisms, and interaction networks. Recombinant TKL8 serves as a vital tool for *in vitro* assays, structural studies (e.g., X-ray crystallography), and inhibitor screening. Challenges remain in fully characterizing its physiological roles, partly due to functional redundancy within the kinase family. Nonetheless, advancements in recombinant protein technology and CRISPR-based functional genomics are accelerating discoveries, highlighting TKL8's relevance in both cellular homeostasis and disease contexts.