| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TKL7 |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TKL7重组蛋白的模拟参考文献示例(内容为虚构，仅供参考)：
1. **文献名称**: "Recombinant TKL7 Protein Expression and Its Role in Wnt Signaling Pathway"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了具有生物活性的TKL7重组蛋白，证实其通过调控Wnt/β-catenin信号通路影响结肠癌细胞的迁移和侵袭能力，为靶向治疗提供了实验依据。
2. **文献名称**: "Structural Characterization of TKL7 Kinase Domain by X-ray Crystallography"
**作者**: Smith JA, et al.
**摘要**: 通过X射线晶体学解析了TKL7重组蛋白激酶结构域的三维结构，揭示了其独特的ATP结合位点构象，为设计特异性小分子抑制剂奠定结构基础。
3. **文献名称**: "TKL7 Recombinant Protein Enhances Neuronal Differentiation in vitro"
**作者**: Gupta R, et al.
**摘要**: 研究发现，外源性添加TKL7重组蛋白可显著促进神经干细胞向多巴胺能神经元分化，其机制可能与激活ERK/MAPK通路相关，提示其在神经再生中的潜在应用价值。
4. **文献名称**: "Development of a TKL7-Specific ELISA Kit Using Recombinant Antigen"
**作者**: Tanaka H, et al.
**摘要**: 基于重组TKL7蛋白制备的高灵敏度ELISA检测试剂盒，成功应用于肝癌患者血清样本中TKL7的定量分析，证实其作为新型生物标志物的临床潜力。
注：以上文献为模拟内容，实际研究中请通过PubMed或Web of Science等平台检索真实发表的论文。
**Background of TKL7 Recombinant Protein**
TKL7 (Tyrosine Kinase-Like 7) is a protein of interest in biomedical research due to its structural and functional similarities to tyrosine kinases, a class of enzymes critical for regulating cellular signaling pathways. While not a canonical tyrosine kinase, TKL7 belongs to the tyrosine kinase-like (TKL) subgroup within the broader protein kinase superfamily. Its precise biological role remains under investigation, but studies suggest involvement in cellular processes such as proliferation, differentiation, and apoptosis, potentially through interactions with signaling cascades like MAPK or Wnt pathways.
Recombinant TKL7 protein is engineered using molecular cloning techniques, often expressed in heterologous systems like *E. coli*, insect cells, or mammalian cell lines to ensure proper folding and post-translational modifications. This engineered protein enables researchers to study TKL7's biochemical properties, substrate specificity, and interactions in controlled settings. Its recombinant form is particularly valuable for structural studies (e.g., X-ray crystallography or cryo-EM), inhibitor screening, and functional assays to elucidate its role in disease contexts.
Emerging evidence links dysregulated TKL7 expression to pathological conditions, including cancers and inflammatory disorders. For example, aberrant TKL7 activity has been observed in certain tumors, suggesting its potential as a therapeutic target or diagnostic biomarker. Additionally, its role in immune modulation has sparked interest in autoimmune disease research.
Despite progress, challenges remain in fully characterizing TKL7’s mechanistic contributions and regulatory networks. Ongoing research aims to clarify its physiological functions and explore its therapeutic applicability, leveraging recombinant TKL7 as a tool for drug discovery and mechanistic studies. This protein thus represents a bridge between basic kinase biology and translational medical innovation.
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