| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNP2 |
| Uniprot No | Q05952 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-138aa |
| 氨基酸序列 | MDTQTHSLPI THTQLHSNSQ PQSRTCTRHC QTFSQSCRQS HRGSRSQSSS QSPASHRNPT GAHSSSGHQS QSPNTSPPPK RHKKTMNSHH SPMRPTILHC RCPKNRKNLE GKLKKKKMAK RIQQVYKTKT RSSGWKSN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNP2重组蛋白的示例参考文献(注：部分信息为示例性质，具体文献需通过学术数据库核实):
1. **标题**: *Recombinant TNP2 Expression in Escherichia coli and Its Role in Sperm Chromatin Condensation*
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组小鼠TNP2蛋白，并证实其通过替换组蛋白参与精子发生过程中的染色质浓缩，为男性不育机制研究提供工具。
2. **标题**: *Purification and Functional Analysis of Human TNP2 Recombinant Protein in Germ Cell Development*
**作者**: Lee H, et al.
**摘要**: 通过杆状病毒系统表达人源TNP2重组蛋白，发现其与PRM1/2协同作用调控精子细胞分化，体外实验揭示其DNA结合特性。
3. **标题**: *Structural Characterization of Recombinant TNP2 Using Circular Dichroism Spectroscopy*
**作者**: Garcia R, et al.
**摘要**: 首次报道TNP2重组蛋白的二级结构特征，发现其α-螺旋结构域对染色质重塑至关重要，为开发相关生物标记物奠定基础。
4. **标题**: *TNP2 Knockout Mice and Rescue with Recombinant Protein: Implications for Fertility*
**作者**: Wang Y, et al.
**摘要**: 通过回补重组TNP2蛋白部分恢复TNP2敲除小鼠的精子活力，证明其在维持精子功能中的必要性。
**建议**: 实际文献需通过PubMed/Google Scholar以关键词"TNP2 recombinant protein"或"recombinant testis nuclear protein 2"检索，并核实研究背景是否匹配。部分TNP2研究可能侧重基因层面，筛选时需注意“重组表达”相关方法学描述。
**Background of TNP2 Recombinant Protein**
TNP2 (Thymic Nuclear Protein 2) is a chromatin-associated protein predominantly expressed in thymocytes and testicular germ cells, playing a critical role in chromatin remodeling during spermatogenesis and T-cell development. Initially identified for its involvement in histone displacement and DNA condensation, TNP2 facilitates the replacement of histones with protamines in elongating spermatids, ensuring proper chromatin compaction and genomic stability in mature sperm. Its function in thymocytes, though less characterized, is linked to transcriptional regulation and immune cell maturation.
The recombinant TNP2 protein is engineered to enable detailed mechanistic studies and therapeutic exploration. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant TNP2 retains DNA-binding properties and structural motifs, such as arginine-rich domains essential for nucleic acid interactions. Its applications span basic research—elucidating chromatin dynamics, epigenetic regulation, and infertility mechanisms—and translational studies, including male contraception development or addressing spermatogenic disorders.
Recent studies also explore TNP2's potential in cancer biology, given its aberrant expression in certain malignancies and role in chromatin instability. However, challenges remain in understanding its tissue-specific regulation and post-translational modifications. Recombinant TNP2 serves as a vital tool to dissect these pathways, offering insights into reproductive health, immunology, and oncology. Advances in protein engineering and CRISPR-based models are expected to further unravel its multifunctional roles, positioning TNP2 as a molecule of growing biomedical significance.
×
