| 纯度 | >90%SDS-PAGE. |
| 种属 | E. coli |
| 靶点 | TKL3 |
| Uniprot No | P30249 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于TKL3重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**: "Structural and Functional Characterization of TKL3 Recombinant Protein in Cancer Signaling"
**作者**: Zhang, L., et al.
**摘要**: 研究通过大肠杆菌系统表达TKL3重组蛋白，解析其晶体结构并验证其在MAPK信号通路中的调控作用，发现TKL3通过磷酸化下游靶点抑制肿瘤细胞迁移。
2. **文献名称**: "TKL3 Recombinant Protein as a Novel Therapeutic Target for Autoimmune Diseases"
**作者**: Kim, S., & Patel, R.
**摘要**: 利用哺乳动物细胞表达体系获得高纯度TKL3重组蛋白，实验证实其可特异性结合IL-17受体，在类风湿性关节炎小鼠模型中显著降低炎症因子水平。
3. **文献名称**: "Optimization of TKL3 Recombinant Protein Expression in Pichia pastoris"
**作者**: García, M., et al.
**摘要**: 报道在毕赤酵母中优化TKL3重组蛋白的分泌表达条件，通过糖基化修饰提高蛋白稳定性，为大规模生产具有生物活性的TKL3提供可行方案。
**注意**：以上内容为模拟生成，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(可用关键词：TKL3 recombinant protein/kinase/therapeutic application)。
**Background of TKL3 Recombinant Protein**
TKL3 (Tyrosine Kinase-Like 3) is a member of the tyrosine kinase-like (TKL) protein family, which shares structural similarities with tyrosine kinases but lacks canonical kinase activity. It is implicated in regulating cellular signaling pathways, particularly those influencing cell proliferation, differentiation, and apoptosis. TKL3 is thought to interact with components of the MAPK (mitogen-activated protein kinase) and JAK-STAT (Janus kinase-signal transducer and activator of transcription) pathways, though its exact molecular mechanisms remain under investigation. Dysregulation of TKL3 has been linked to pathological conditions, including cancer, fibrosis, and inflammatory diseases, highlighting its potential as a therapeutic target.
Recombinant TKL3 protein is produced using biotechnological methods, often via expression in *E. coli* or mammalian cell systems, followed by purification through affinity chromatography. This engineered protein retains functional domains critical for binding partners or substrates, enabling its use in *in vitro* assays, structural studies, and drug discovery. Researchers employ TKL3 recombinant protein to study its role in signaling cascades, screen for inhibitors or activators, and validate interactions with other biomolecules.
Current research focuses on elucidating TKL3's tissue-specific functions, post-translational modifications (e.g., phosphorylation), and crosstalk with other pathways. Its involvement in epithelial-mesenchymal transition (EMT) and immune modulation further underscores its relevance in developmental biology and disease pathogenesis. As a tool, recombinant TKL3 accelerates both basic research and translational applications, offering insights into novel diagnostic or therapeutic strategies.
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