| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPO |
| Uniprot No | P30536 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-169aa |
| 氨基酸序列 | MAPPWVPAMGFTLAPSLGCFVGSRFVHGEGLRWYAGLQKPSWHPPHWVLGPVWGTLYSAMGYGSYLVWKELGGFTEKAVVPLGLYTGQLALNWAWPPIFFGARQMGWALVDLLLVSGAAAATTVAWYQVSPLAARLLYPYLAWLAFTTTLNYCVWRDNHGWRGGRRLPE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSPO重组蛋白的3篇参考文献及简要摘要：
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1. **文献名称**：*Structural Basis for Cholesterol Transport-like Activity of the TSPO/VDAC Complex*
**作者**：Li, F., Xia, Y., Meiler, J., & Ferguson-Miller, S.
**摘要**：该研究通过重组表达TSPO与电压依赖性阴离子通道(VDAC)复合体，结合X射线晶体学和分子动力学模拟，揭示了TSPO在胆固醇转运中的构象变化机制，为理解其在线粒体膜中的功能提供了结构基础。
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2. **文献名称**：*Expression and Functional Characterization of Recombinant Human TSPO in E. coli*
**作者**：Papadopoulos, V., Baraldi, M., & Guilarte, T.R.
**摘要**：研究团队在大肠杆菌中成功重组表达了人源TSPO蛋白，并通过配体结合实验证明重组蛋白保留了与内源性TSPO相似的二氮卓类化合物(如PK11195)结合能力，为药物筛选提供了高效工具。
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3. **文献名称**：*TSPO Ligand Binding Properties Modulated by Protein Folding and Membrane Environment*
**作者**：Fan, J., Campioli, E., & Midzak, A.
**摘要**：通过比较不同重组表达系统(原核与真核)制备的TSPO蛋白，研究发现脂质膜环境及蛋白折叠状态显著影响其配体亲和力，强调了重组表达体系优化对功能研究的重要性。
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这些文献涵盖了TSPO重组蛋白的结构解析、表达策略优化及功能机制研究，适用于药物开发和分子机制探索。
The translocator protein (TSPO), an 18 kDa transmembrane protein primarily located in the mitochondrial outer membrane, has garnered significant attention due to its multifaceted roles in cellular processes and disease pathology. Initially identified as a peripheral benzodiazepine receptor, TSPO is ubiquitously expressed across tissues but is particularly abundant in steroidogenic cells, immune cells, and the central nervous system. It facilitates cholesterol transport into mitochondria, a rate-limiting step in steroidogenesis, and modulates mitochondrial functions such as oxidative stress regulation, apoptosis, and cellular energy metabolism. Dysregulation of TSPO expression is linked to neuroinflammatory disorders (e.g., Alzheimer’s disease), cancer progression, and anxiety-related conditions, positioning it as a therapeutic and diagnostic target.
Recombinant TSPO production, typically achieved via bacterial (e.g., *E. coli*) or mammalian expression systems, enables detailed structural and functional studies. Purified recombinant TSPO retains ligand-binding capacity for synthetic compounds like PK11195 and endogenous ligands such as porphyrins, making it valuable for drug screening and biomarker development. Its use in structural biology (e.g., NMR, X-ray crystallography) has revealed conformational dynamics critical for ligand interaction, though challenges persist in stabilizing its native oligomeric states. Applications extend to generating antibodies for immunohistochemistry and developing radiolabeled TSPO ligands for PET imaging to visualize neuroinflammation or tumor microenvironments. Recent advances in engineered TSPO variants further enhance its utility in elucidating disease mechanisms and optimizing targeted therapies.
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