| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIRE |
| Uniprot No | O43918 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-545aa |
| 氨基酸序列 | MATDAALRRL LRLHRTEIAV AVDSAFPLLH ALADHDVVPE DKFQETLHLK EKEGCPQAFH ALLSWLLTQD STAILDFWRV LFKDYNLERY GRLQPILDSF PKDVDLSQPR KGRKPPAVPK ALVPPPRLPT KRKASEEARA AAPAALTPRG TASPGSQLKA KPPKKPESSA EQQRLPLGNG IQTMSASVQR AVAMSSGDVP GARGAVEGIL IQQVFESGGS KKCIQVGGEF YTPSKFEDSG SGKNKARSSS GPKPLVRAKG AQGAAPGGGE ARLGQQGSVP APLALPSDPQ LHQKNEDECA VCRDGGELIC CDGCPRAFHL ACLSPPLREI PSGTWRCSSC LQATVQEVQP RAEEPRPQEP PVETPLPPGL RSAGEEVRGP PGEPLAGMDT TLVYKHLPAP PSAAPLPGLD SSALHPLLCV GPEGQQNLAP GARCGVCGDG TDVLRCTHCA AAFHWRCHFP AGTSRPGTGL RCRSCSGDVT PAPVEGVLAP SPARLAPGPA KDDTASHEPA LHRDDLESLL SEHTFDGILQ WAIQSMARPA APFPS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AIRE重组蛋白的3篇代表性文献，按研究内容分类整理：
1. **《AIRE structure and function in thymic epithelial cells》**
作者：Anderson, M. S., & Su, M. A.
摘要：解析AIRE蛋白结构域及其在胸腺上皮细胞中促进组织特异性抗原表达的分子机制，通过重组AIRE蛋白实验验证其DNA结合与转录调控功能。
2. **《Recombinant AIRE enhances antigen presentation in vitro》**
作者：Peterson, P., et al.
摘要：研究重组AIRE蛋白在体外模型中对MHC-II类抗原呈递的增强作用，揭示其通过染色质重塑机制促进外周组织抗原的异位表达。
3. **《Structural basis of AIRE-mediated autoimmunity》**
作者：Kisand, K., & Boe Wolff, A. S.
摘要：通过X射线晶体学解析重组AIRE蛋白的CARD结构域三维结构，阐明其突变如何导致APECED综合征的分子病理机制。
注：上述文献信息为领域内典型研究方向概括，实际引用请核对具体论文。建议通过PubMed等平台用关键词"AIRE recombinant protein structure/function"检索最新文献，重点关注Nature Immunology、Immunity等期刊近5年论文。
**Background of AIRE Recombinant Protein**
The **Autoimmune Regulator (AIRE)** protein is a critical transcriptional regulator primarily expressed in medullary thymic epithelial cells (mTECs). Its discovery stemmed from studies on **Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy (APECED)**, a rare monogenic autoimmune disorder caused by mutations in the *AIRE* gene. AIRE plays a pivotal role in central immune tolerance by promoting the expression of tissue-restricted antigens (TRAs) in the thymus. This process enables developing T-cells to encounter self-antigens, eliminating autoreactive T-cells and fostering regulatory T-cell development, thereby preventing autoimmune reactions.
**AIRE recombinant protein** refers to the engineered form of AIRE produced via molecular cloning and expression systems (e.g., bacterial, mammalian, or insect cells). Recombinant AIRE retains key functional domains, including the **homogenously staining region (HSR)** and **plant-homeodomain (PHD) fingers**, which mediate its interaction with chromatin and transcriptional machinery. This protein is widely used in *in vitro* studies to dissect AIRE's mechanisms in immune tolerance, gene regulation, and epigenetic modulation.
Research applications include modeling AIRE-deficient conditions, screening for therapeutic compounds, and studying its synergy with other factors (e.g., chromatin remodelers). Additionally, recombinant AIRE aids in investigating *AIRE* mutations linked to APECED and exploring potential gene therapies. Beyond autoimmunity, AIRE's role in cancer immunity and infectious diseases is an emerging focus.
Overall, AIRE recombinant protein serves as a vital tool for unraveling immune tolerance mechanisms and developing interventions for autoimmune disorders and immune dysregulation syndromes.
×
