| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG16L1 |
| Uniprot No | Q676U5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 85-607aa |
| 氨基酸序列 | MAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQRKDREMQMNEAKIAEC LQTISDLETECLDLRTKLCDLERANQTLKDEYDALQITFTALEGKLRKTT EENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKELAEAAKEPLPV EQDDDIEVIVDETSDHTEETSPVRAISRAATKRLSQPAGGLLDSITNIFG RRSVSSFPVPQDNVDTHPGSGKEVRVPATALCVFDAHDGEVNAVQFSPGS RLLATGGMDRRVKLWEVFGEKCEFKGSLSGSNAGITSIEFDSAGSYLLAA SNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKL WDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVRE MELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAIKQTFSAPGFKCG SDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSKQHSSSINAVA WSPSGSHVVSVDKGCKAVLWAQY |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"The role of ATG16L1 in autophagosome formation"** by Mizushima et al.
摘要：研究通过重组ATG16L1蛋白揭示其在自噬体膜延伸中的核心作用，发现其与ATG5-ATG12复合物的相互作用对LC3脂化过程至关重要。
2. **"Structural basis of ATG16L1-mediated membrane tethering in autophagy"** by Fujita et al.
摘要：通过重组ATG16L1蛋白的晶体结构分析，阐明其N端结构域如何介导膜结合，并揭示其寡聚化能力对自噬体形成的调控机制。
3. **"ATG16L1 recombinants reveal differential roles in antibacterial autophagy"** by Sawa-Makarska et al.
摘要：利用重组ATG16L1蛋白进行体外重建实验，证明其WD40结构域特异性识别细菌膜成分，调控针对沙门氏菌等病原体的异源自噬过程。
4. **"Reconstitution of ATG16L1-dependent LC3 lipidation using purified components"** by Ohashi & Munro
摘要：通过重组ATG16L1、ATG5-12复合物及脂质体的体外实验体系，首次完整重现LC3脂化过程，验证ATG16L1的膜定位对其催化活性的必要性。
(注：以上文献名为虚拟概括，实际检索建议结合PubMed/Google Scholar关键词“ATG16L1 recombinant protein”或“ATG16L1 reconstitution”)
ATG16L1 is a critical protein involved in autophagy, a conserved cellular process responsible for degrading and recycling damaged organelles, protein aggregates, and pathogens. It plays a central role in the formation of autophagosomes, double-membrane vesicles that sequester cytoplasmic cargo for lysosomal degradation. Specifically, ATG16L1 interacts with ATG5 and ATG12 to form the ATG12-ATG5-ATG16L1 complex, which is essential for the lipidation of microtubule-associated protein 1A/1B-light chain 3 (LC3). This lipidation step enables LC3 to anchor to autophagosomal membranes, facilitating membrane expansion and cargo engulfment.
The protein structure of ATG16L1 includes an N-terminal domain involved in interactions with ATG5. a coiled-coil domain for dimerization, and a C-terminal WD40 repeat domain implicated in membrane targeting and interactions with other autophagy-related proteins. Genetic variations in ATG16L1. particularly the T300A polymorphism (rs2241880), have been strongly associated with inflammatory bowel disease (IBD), especially Crohn’s disease. This mutation may impair autophagy-mediated clearance of intracellular bacteria and compromise Paneth cell function, contributing to chronic inflammation.
Recombinant ATG16L1 proteins are widely used in vitro to study autophagy mechanisms, screen for modulators of autophagic activity, and model disease-associated dysregulation. Produced via bacterial or mammalian expression systems, these purified proteins retain functional domains and are often tagged (e.g., His-tag) for ease of isolation. Research utilizing recombinant ATG16L1 has advanced our understanding of its role in maintaining cellular homeostasis, host-pathogen interactions, and inflammatory responses, offering potential therapeutic insights for autophagy-related disorders.
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