| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPP2 |
| Uniprot No | Q14168 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-576aa |
| 氨基酸序列 | MPVAATNSET AMQQVLDNLG SLPSATGAAE LDLIFLRGIM ESPIVRSLAK VIMVLWFMQQ NVFVPMKYML KYFGAHERLE ETKLEAVRDN NLELVQEILR DLAHVAEQSS TAAELAHILQ EPHFQSLLET HDSVASKTYE TPPPSPGLDP TFSNQPVPPD AVRMVGIRKT AGEHLGVTFR VEGGELVIAR ILHGGMVAQQ GLLHVGDIIK EVNGQPVGSD PRALQELLRN ASGSVILKIL PSYQEPHLPR QVFVKCHFDY DPARDSLIPC KEAGLRFNAG DLLQIVNQDD ANWWQACHVE GGSAGLIPSQ LLEEKRKAFV KRDLELTPNS GTLCGSLSGK KKKRMMYLTT KNAEFDRHEL LIYEEVARMP PFRRKTLVLI GAQGVGRRSL KNKLIMWDPD RYGTTVPYTS RRPKDSEREG QGYSFVSRGE MEADVRAGRY LEHGEYEGNL YGTRIDSIRG VVAAGKVCVL DVNPQAVKVL RTAEFVPYVV FIEAPDFETL RAMNRAALES GISTKQLTEA DLRRTVEESS RIQRGYGHYF DLCLVNSNLE RTFRELQTAM EKLRTEPQWV PVSWVY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPP2重组蛋白的示例性参考文献(注：文献为假设性示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*"Recombinant MPP2 Expression in Insect Cells and Its Role in Synaptic Scaffolding"*
**作者**：Kimura T, et al.
**摘要**：本研究利用杆状病毒-昆虫细胞系统成功表达并纯化了重组MPP2蛋白，证实其通过与PSD-95和钾离子通道蛋白的相互作用参与突触后支架结构的组装，揭示了MPP2在神经信号传递中的关键作用。
2. **文献名称**：*"Structural Characterization of MPP2 Using Cryo-EM and X-ray Crystallography"*
**作者**：Wang L, et al.
**摘要**：通过冷冻电镜和X射线晶体学解析了重组MPP2蛋白的三维结构，发现其PDZ结构域特异性结合特定靶蛋白的C端序列，为设计靶向MPP2相互作用的药物提供了结构基础。
3. **文献名称**：*"Functional Analysis of MPP2 in Epithelial Tight Junction Formation"*
**作者**：Gonzalez A, et al.
**摘要**：通过大肠杆菌表达系统获得重组MPP2蛋白，体外实验证明其与ZO-1和Claudin蛋白的互作，调控上皮细胞紧密连接的形成，提示MPP2在屏障功能中的潜在应用。
4. **文献名称**：*"Optimization of MPP2 Soluble Expression in E. coli for Biochemical Studies"*
**作者**：Chen R, et al.
**摘要**：系统优化了MPP2重组蛋白在大肠杆菌中的可溶性表达条件，并验证其与脂筏标记蛋白的共定位，为后续膜蛋白功能研究提供了高效制备方案。
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**建议**：实际研究中可访问PubMed或Web of Science，以关键词“MPP2 recombinant protein”“MPP2 expression”等检索最新文献。MPP2的研究多集中于细胞极性、神经突触及上皮屏障等领域，重组蛋白技术常服务于其互作蛋白筛选或结构功能解析。
**Background of MPP2 Recombinant Protein**
The membrane palmitoylated protein 2 (MPP2), a member of the membrane-associated guanylate kinase (MAGUK) family, plays a critical role in maintaining cell polarity and organizing junctional complexes in epithelial and neuronal cells. MAGUK proteins are scaffold molecules that facilitate the assembly of multiprotein complexes at specialized membrane domains, such as tight junctions and synapses. MPP2 contains conserved protein-binding domains, including a PDZ domain, an SH3 domain, and a guanylate kinase (GK)-like domain, enabling interactions with cytoskeletal components, receptors, and signaling proteins.
Recombinant MPP2 protein is produced using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper folding and post-translational modifications. Its recombinant form allows researchers to study MPP2’s molecular functions *in vitro*, including its role in stabilizing cell-cell adhesion, regulating ion channel activity, and participating in intracellular signaling pathways. MPP2 interacts with proteins like cadherins, CRB3. and voltage-gated potassium channels, linking cell adhesion to polarity and electrical signaling.
Dysregulation of MPP2 has been implicated in neurodevelopmental disorders and cancer metastasis, highlighting its biomedical relevance. Recombinant MPP2 is widely used in structural studies, binding assays, and drug discovery to explore mechanisms underlying tissue integrity and disease. Quality control involves validation via SDS-PAGE, Western blotting, and mass spectrometry to confirm purity and bioactivity. Ongoing research aims to elucidate its tissue-specific roles and therapeutic potential.
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