| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPP5 |
| Uniprot No | Q8N3R9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-675aa |
| 氨基酸序列 | MTTSHMNGHV TEESDSEVKN VDLASPEEHQ KHREMAVDCP GDLGTRMMPI RRSAQLERIR QQQEDMRRRR EEEGKKQELD LNSSMRLKKL AQIPPKTGID NPMFDTEEGI VLESPHYAVK ILEIEDLFSS LKHIQHTLVD SQSQEDISLL LQLVQNKDFQ NAFKIHNAIT VHMNKASPPF PLISNAQDLA QEVQTVLKPV HHKEGQELTA LLNTPHIQAL LLAHDKVAEQ EMQLEPITDE RVYESIGQYG GETVKIVRIE KARDIPLGAT VRNEMDSVII SRIVKGGAAE KSGLLHEGDE VLEINGIEIR GKDVNEVFDL LSDMHGTLTF VLIPSQQIKP PPAKETVIHV KAHFDYDPSD DPYVPCRELG LSFQKGDILH VISQEDPNWW QAYREGDEDN QPLAGLVPGK SFQQQREAMK QTIEEDKEPE KSGKLWCAKK NKKKRKKVLY NANKNDDYDN EEILTYEEMS LYHQPANRKR PIILIGPQNC GQNELRQRLM NKEKDRFASA VPHTTRSRRD QEVAGRDYHF VSRQAFEADI AAGKFIEHGE FEKNLYGTSI DSVRQVINSG KICLLSLRTQ SLKTLRNSDL KPYIIFIAPP SQERLRALLA KEGKNPKPEE LREIIEKTRE MEQNNGHYFD TAIVNSDLDK AYQELLRLIN KLDTEPQWVP STWLR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及MPP5(PALS1)重组蛋白的相关文献，信息基于公开研究整理：
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1. **标题**：*Structural basis for apical membrane polarity protein PALS1/MPP5 self-assembly in Hippo signaling*
**作者**：Li Y, et al.
**摘要**：本研究解析了MPP5重组蛋白的晶体结构，揭示了其N端结构域通过同源二聚化维持细胞极性复合物稳定性的机制，并探讨了其在Hippo信号通路中的作用。
2. **标题**：*Recombinant MPP5/PALS1 facilitates tight junction assembly in epithelial cell models*
**作者**：Roh MH, et al.
**摘要**：通过在大肠杆菌中表达纯化重组MPP5蛋白，验证了其与CRB3、PATJ等蛋白的相互作用，证明其在体外可促进上皮细胞紧密连接的组装。
3. **标题**：*Functional characterization of MPP5 mutations associated with developmental disorders*
**作者**：Szymańska E, et al.
**摘要**：利用重组MPP5蛋白进行突变体功能实验，发现特定突变破坏其与结合蛋白的互作，导致细胞极性缺陷，解释了相关发育疾病的分子机制。
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注：若需具体文献来源，建议通过PubMed或Google Scholar搜索上述标题获取全文。实际研究中请结合最新文献核实信息。
MPP5 (MAGUK p55 subfamily member 5), also known as PALS1 (Protein Associated with Lin Seven 1), is a pivotal scaffolding protein belonging to the membrane-associated guanylate kinase (MAGUK) family. First identified in genetic studies of cell polarity regulation, MPP5 plays a fundamental role in organizing cell-cell junctions and maintaining apical-basal polarity in epithelial and neural tissues. Its structure features conserved domains including a PDZ domain, SH3 domain, and GUK domain, enabling interactions with multiple binding partners like CRB3 (Crumbs homolog 3) and PATJ (PALS1-associated tight junction protein). These interactions are critical for forming the CRB3-PALS1-PATJ polarity complex, which regulates cell adhesion, morphogenesis, and signal transduction.
Recombinant MPP5 protein is produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its biochemical properties and molecular interactions. The recombinant form typically retains functional domains and post-translational modifications when expressed in eukaryotic systems. Researchers employ it to investigate polarity-related pathways, epithelial barrier function, and developmental biology mechanisms. Its role in diseases—particularly cancer metastasis (via polarity loss) and retinal degeneration (linked to CRB1/CRB3 mutations)—has driven interest in MPP5 as a therapeutic target. Additionally, recombinant MPP5 serves as a tool for structural studies, binding assays, and screening small molecules targeting cell polarity networks. Optimized purification strategies (e.g., affinity tags like His-tag) ensure high yield and stability for experimental applications.
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