| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRH1 |
| Uniprot No | P35367 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 211-416aa |
| 氨基酸序列 | AKIYKAVRQHCQHRELINRSLPSFSEIKLRPENPKGDAKKPGKESPWEVLKRKPKDAGGGSVLKSPSQTPKEMKSPVVFSQEDDREVDKLYCFPLDIVHMQAAAEGSSRDYVAVNRSHGQLKTDEQGLNTHGASEISEDQMLGDSQSFSRTDSDTTTETAPGKGKLRSGSNTGLDYIKFTWKRLRSHSRQYVSGLHMNRERKAAKQ |
| 预测分子量 | 27.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于HRH1(组胺受体H1)重组蛋白研究的虚构参考文献示例(实际文献请通过学术平台检索)：
---
1. **标题**：*Expression and Purification of Recombinant Human Histamine H1 Receptor in Insect Cells*
**作者**：Smith J, et al.
**摘要**：研究通过杆状病毒-昆虫细胞系统表达HRH1重组蛋白，优化了纯化条件，验证其与抗组胺药物的结合活性，为体外药理学研究提供工具。
2. **标题**：*Structural Insights into HRH1 Signaling Mechanism via Cryo-EM Analysis*
**作者**：Wang L, et al.
**摘要**：利用冷冻电镜解析HRH1重组蛋白与拮抗剂结合的高分辨率结构，揭示受体激活的构象变化，为靶向药物设计提供结构基础。
3. **标题**：*Functional Characterization of HRH1 Expressed in HEK293 Cells for Drug Screening*
**作者**：Garcia R, et al.
**摘要**：在HEK293细胞中稳定表达HRH1重组蛋白，通过钙流实验评估其信号转导功能，建立高通量抗过敏药物筛选模型。
4. **标题**：*Role of HRH1 in Allergic Inflammation: Studies with Recombinant Protein Knockdown Models*
**作者**：Kim S, et al.
**摘要**：通过重组HRH1蛋白和siRNA技术证明其在肥大细胞中调控IL-6和TNF-α分泌的机制，提示其在过敏性疾病中的治疗潜力。
---
**注意**：以上为示例文献，实际研究需通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词(如 "Recombinant HRH1 protein" "Histamine H1 receptor expression")。真实文献可能涉及以下方向：
- 重组表达系统(昆虫/哺乳动物细胞)
- 受体-配体相互作用分析
- 结构生物学研究(X射线、冷冻电镜)
- 疾病模型中的功能验证
**Background of HRH1 Recombinant Protein**
The histamine H1 receptor (HRH1) is a G protein-coupled receptor (GPCR) that plays a central role in mediating allergic and inflammatory responses. Upon activation by histamine, HRH1 triggers intracellular signaling cascades, primarily via Gq/11 proteins, leading to pathways such as phospholipase C (PLC) activation, calcium mobilization, and NF-κB signaling. This receptor is expressed widely in tissues, including smooth muscle, vascular endothelium, and the central nervous system, and is implicated in conditions like allergic rhinitis, asthma, and itching.
HRH1 recombinant protein refers to a lab-engineered version of the receptor, produced using heterologous expression systems (e.g., mammalian, insect, or bacterial cells). Recombinant HRH1 retains the structural and functional characteristics of the native receptor, including its seven transmembrane domains, ligand-binding sites, and coupling to downstream signaling effectors. Its production often involves tagging (e.g., His-tag, FLAG-tag) to facilitate purification and detection.
This recombinant tool has become indispensable for studying HRH1's molecular mechanisms, ligand interactions, and signaling dynamics. It enables high-throughput drug screening for antihistamines and inverse agonists, structural studies (e.g., X-ray crystallography, cryo-EM), and the development of targeted therapies for allergic and inflammatory disorders. Additionally, recombinant HRH1 aids in deciphering its role in non-allergic processes, such as circadian rhythm regulation and neurotransmission. By providing a standardized, scalable source of the receptor, recombinant HRH1 accelerates both basic research and therapeutic innovation.
×
