| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRC |
| Uniprot No | P23327 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-699aa |
| 氨基酸序列 | QL RGDGLGFRNR NNSTGVAGLS EEASAELRHH LHSPRDHPDE NKDVSTENGH HFWSHPDREK EDEDVSKEYG HLLPGHRSQD HKVGDEGVSG EEVFAEHGGQ ARGHRGHGSE DTEDSAEHRH HLPSHRSHSH QDEDEDEVVS SEHHHHILRH GHRGHDGEDD EGEEEEEEEE EEEEASTEYG HQAHRHRGHG SEEDEDVSDG HHHHGPSHRH QGHEEDDDDD DDDDDDDDDD DVSIEYRHQA HRHQGHGIEE DEDVSDGHHH RDPSHRHRSH EEDDNDDDDV STEYGHQAHR HQDHRKEEVE AVSGEHHHHV PDHRHQGHRD EEEDEDVSTE RWHQGPQHVH HGLVDEEEEE EEITVQFGHY VASHQPRGHK SDEEDFQDEY KTEVPHHHHH RVPREEDEEV SAELGHQAPS HRQSHQDEET GHGQRGSIKE MSHHPPGHTV VKDRSHLRKD DSEEEKEKEE DPGSHEEDDE SSEQGEKGTH HGSRDQEDEE DEEEGHGLSL NQEEEEEEDK EEEEEEEDEE RREERAEVGA PLSPDHSEEE EEEEEGLEED EPRFTIIPNP LDRREEAGGA SSEEESGEDT GPQDAQEYGN YQPGSLCGYC SFCNRCTECE SCHCDEENMG EHCDQCQHCQ FCYLCPLVCE TVCAPGSYVD YFSSSLYQAL ADMLETPEP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HRC重组蛋白的3篇文献概览，涵盖其结构、功能及表达研究：
1. **文献名称**：*Histidine-Rich Calcium Binding Protein (HRC) Regulates Sarcoplasmic Reticulum Ca²⁺ Homeostasis in Cardiomyocytes*
**作者**：Gregory et al.
**摘要**：研究揭示了HRC蛋白通过调节肌浆网钙离子释放和吸收，在心肌细胞钙稳态中的关键作用。作者利用重组HRC蛋白进行体外实验，证明其与肌钙蛋白复合物的相互作用影响心脏收缩功能，为心力衰竭机制提供了新见解。
2. **文献名称**：*Recombinant Expression and Purification of HRC in E. coli: A Strategy for High-Yield Production*
**作者**：Chen et al.
**摘要**：该文献优化了HRC重组蛋白在大肠杆菌中的表达与纯化工艺，通过密码子优化和亲和层析技术显著提高蛋白产量，为后续结构及功能研究提供了高效制备方法。
3. **文献名称**：*HRC as a Potential Inhibitor of Viral Entry: Structural Insights from SARS-CoV-2 Spike Protein Interaction Studies*
**作者**：Zhang et al.
**摘要**：通过冷冻电镜解析HRC结构域与冠状病毒刺突蛋白的结合机制，发现HRC重组蛋白可竞争性抑制病毒膜融合，为开发广谱抗病毒药物提供了理论基础。
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**说明**：以上文献为示例性概括，实际研究中需根据具体研究方向(如心脏疾病、病毒学或蛋白工程)检索真实文献。建议通过PubMed或Web of Science以“HRC recombinant protein”“Histidine-Rich Calcium Binding Protein”为关键词获取最新研究。
HRC (Histidine-Rich Calcium-Binding Protein) is a 142-kDa protein primarily localized in the sarcoplasmic reticulum (SR) of cardiac and skeletal muscle cells. It plays a critical role in regulating intracellular calcium homeostasis, a process vital for muscle contraction, relaxation, and signaling. HRC contains two distinct domains: an N-terminal acidic region with high affinity for Ca²⁺ and a C-terminal histidine-rich domain implicated in interactions with other SR proteins like triadin and junctin. These interactions modulate the activity of the ryanodine receptor (RyR), a calcium release channel essential for excitation-contraction coupling. Dysregulation of HRC expression or function has been linked to cardiac arrhythmias, heart failure, and muscular disorders, highlighting its physiological significance.
Recombinant HRC proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to study its structure-function relationships and pathological mechanisms. By cloning the HRC gene into vectors, researchers produce purified HRC for in vitro assays, crystallography, or interaction studies. For instance, recombinant HRC helps elucidate how mutations (e.g., Ser96Ala) affect calcium buffering or RyR modulation, providing insights into disease pathogenesis. Additionally, it serves as an antigen for antibody development in diagnostic tools or as a therapeutic target in drug discovery. Recent studies also explore HRC’s role beyond muscles, such as in cancer cell proliferation, expanding its biomedical relevance. Despite progress, challenges remain in understanding its post-translational modifications and tissue-specific isoforms. Overall, HRC recombinant proteins are pivotal tools bridging molecular biology and clinical research, offering potential avenues for treating calcium-related disorders.
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