| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AA |
| Uniprot No | Q96QV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-131aa |
| 氨基酸序列 | SGRGKQGGK ARAKSKSRSS RAGLQFPVGR IHRLLRKGNY AERIGAGAPV YLAAVLEYLT AEILELAGNA SRDNKKTRII PRHLQLAIRN DEELNKLLGG VTIAQGGVLP NIQAVLLPKK TESHHHKAQS K |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H2AA重组蛋白的虚构参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：**"Recombinant HIST1H2AA Facilitates Nucleosome Assembly and Epigenetic Regulation"**
**作者**：Zhang, L. et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备了高纯度HIST1H2AA重组蛋白，并证明其在体外核小体组装中的关键作用，揭示了其对染色质动态调控及基因沉默的影响。
2. **文献名称**：**"Structural Dynamics of HIST1H2AA in DNA Repair: Insights from Recombinant Protein Models"**
**作者**：Müller, R. & Patel, S.
**摘要**：通过重组HIST1H2AA与DNA损伤标记物(如γH2AX)的互作实验，发现该蛋白在DNA双链断裂修复中可能通过构象变化招募修复因子，为癌症治疗靶点提供新思路。
3. **文献名称**：**"Post-Translational Modifications of Recombinant HIST1H2AA: Implications for Cellular Differentiation"**
**作者**：Kim, J. et al.
**摘要**：利用昆虫细胞表达系统获得重组HIST1H2AA，结合质谱分析发现其乙酰化与甲基化修饰模式在干细胞分化过程中动态变化，提示其表观遗传调控功能。
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**提示**：实际研究中建议通过PubMed或Web of Science以关键词“HIST1H2AA recombinant”或“H2A histone recombinant”检索，重点关注其在染色质重构、疾病模型或表观遗传学领域的应用研究。
**Background of HIST1H2AA Recombinant Protein**
HIST1H2AA is a member of the histone H2A family, a critical component of nucleosomes—the fundamental repeating units of chromatin. Histones play a central role in DNA packaging, gene regulation, and epigenetic modifications. The H2A family includes multiple variants, with HIST1H2AA being a canonical subtype encoded by the HIST1 cluster on chromosome 6. This protein contributes to nucleosome assembly by forming an octamer with other core histones (H2B, H3. and H4), around which DNA wraps to maintain chromatin structure and regulate accessibility for transcription, replication, and repair.
Recombinant HIST1H2AA protein is produced using engineered expression systems (e.g., *E. coli* or yeast) to enable large-scale, high-purity yields for research applications. Unlike native histones, recombinant versions avoid post-translational modifications (PTMs) unless specifically engineered, allowing controlled studies of histone-DNA interactions or PTM effects. HIST1H2AA’s recombinant form is pivotal in dissecting mechanisms of chromatin remodeling, epigenetic inheritance, and DNA damage response.
Research leveraging this protein spans cancer biology, developmental genetics, and neurodegenerative diseases, where histone dysfunction is implicated. For instance, mutations or altered expression of HIST1H2AA may disrupt nucleosome stability, contributing to genomic instability or aberrant gene expression. Additionally, recombinant HIST1H2AA is used in structural studies (e.g., cryo-EM or X-ray crystallography) to resolve nucleosome architecture and in biochemical assays to screen for histone-targeted therapeutics. Its utility underscores the importance of histones in both basic science and translational medicine.
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