| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H1A |
| Uniprot No | Q8IZA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346aa |
| 氨基酸序列 | MAPGSVTSDI SPSSTSTAGS SRSPESEKPG PSHGGVPPGG PSHSSLPVGR RHPPVLRMVL EALQAGEQRR GTSVAAIKLY ILHKYPTVDV LRFKYLLKQA LATGMRRGLL ARPLNSKARG ATGSFKLVPK HKKKIQPRKM APATAPRRAG EAKGKGPKKP SEAKEDPPNV GKVKKAAKRP AKVQKPPPKP GAATEKARKQ GGAAKDTRAQ SGEARKVPPK PDKAMRAPSS AGGLSRKAKA KGSRSSQGDA EAYRKTKAES KSSKPTASKV KNGAASPTKK KVVAKAKAPK AGQGPNTKAA APAKGSGSKV VPAHLSRKTE APKGPRKAGL PIKASSSKVS SQRAE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H1A重组蛋白的3篇参考文献及其摘要内容概括：
1. **标题**：*"Recombinant human histone H1.1: production, purification, and chromatin-binding properties"*
**作者**：Smith J, Doe R, Lee T
**摘要**：研究报道了在大肠杆菌中高效表达并纯化重组人源HIST1H1A蛋白的方法，通过体外染色质结合实验证实其能够增强核小体稳定性，并调控染色质高级结构形成。
2. **标题**：*"Functional characterization of recombinant histone H1.1 in epigenetic regulation"*
**作者**：Garcia S, Chen X, Kim M
**摘要**：利用重组HIST1H1A蛋白探究其在基因沉默中的作用，发现其通过与特定转录因子相互作用抑制启动子活性，提示其在细胞分化和肿瘤发生中的潜在功能。
3. **标题**：*"A novel approach for site-specific modification of recombinant histone H1.1 using chemical biology tools"*
**作者**：Zhang Y, Wang L, Ito T
**摘要**：开发了一种基于点击化学的重组HIST1H1A定点修饰技术，实现了荧光标记和甲基化模拟修饰，为研究组蛋白翻译后修饰的功能提供了新工具。
**备注**：上述文献为模拟示例，实际引用时建议通过PubMed或Google Scholar检索具体文章(关键词：HIST1H1A recombinant protein, H1.1 expression, chromatin binding)。若需开放获取文献，可筛选BioRxiv预印本或PMC免费全文库。
HIST1H1A, a member of the histone H1 family, is a linker histone critical for chromatin organization and epigenetic regulation. Histones are core components of nucleosomes, the repeating units of chromatin, and H1 histones bind to DNA between nucleosomes (linker DNA), facilitating higher-order chromatin compaction. This structural role influences gene accessibility, transcriptional regulation, and genome stability. The HIST1H1A gene encodes the H1.1 isoform, predominantly expressed in somatic cells, and is part of a multi-gene cluster on chromosome 6 in humans.
Recombinant HIST1H1A protein is produced using genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper post-translational modifications. Its production enables detailed studies of chromatin dynamics, histone-DNA interactions, and epigenetic mechanisms. Researchers use recombinant H1.1 to investigate its role in gene silencing, cell differentiation, and responses to DNA damage. Unlike core histones (H2A, H2B, H3. H4), H1 histones exhibit greater sequence variability across isoforms and species, making recombinant proteins valuable for isoform-specific functional analyses.
Structurally, HIST1H1A contains a conserved globular domain flanked by disordered N- and C-terminal tails, which mediate chromatin binding and protein interactions. Dysregulation of H1 histones is linked to cancers, developmental disorders, and autoimmune diseases, driving interest in therapeutic targeting. Recombinant HIST1H1A also serves as a tool for developing chromatin-modifying drugs and studying epigenetic biomarkers. Its application extends to *in vitro* assays, antibody production, and structural studies using techniques like X-ray crystallography or cryo-EM. Overall, recombinant HIST1H1A bridges molecular biology and clinical research, offering insights into chromatin biology and disease mechanisms.
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