| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AB |
| Uniprot No | P04908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-130aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY SERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGR VTIAQGGVLP NIQAVLLPKK TESHHKAKGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H2AB重组蛋白的参考文献示例(内容基于学术研究常见方向，非真实文献，仅供参考)：
---
1. **"Expression and purification of recombinant human HIST1H2AB in Escherichia coli"**
*Authors: Zhang Y, et al.*
摘要：本研究优化了HIST1H2AB基因在大肠杆菌中的重组表达条件，采用亲和层析技术高效纯化蛋白，并通过质谱验证其正确性，为后续功能研究提供高纯度蛋白。
2. **"HIST1H2AB-mediated chromatin remodeling in breast cancer progression"**
*Authors: Lee S, et al.*
摘要：探讨HIST1H2AB在乳腺癌细胞中的异常表达及其对染色质结构的影响，发现其通过调控特定基因的启动子可及性促进肿瘤侵袭性。
3. **"Structural analysis of HIST1H2AB in nucleosome assembly using cryo-EM"**
*Authors: Patel R, et al.*
摘要：利用冷冻电镜解析HIST1H2AB重组蛋白参与的核小体复合物结构，揭示其C端修饰对DNA-组蛋白相互作用的关键调控机制。
4. **"HIST1H2AB acetylation dynamics during cellular differentiation"**
*Authors: Gonzalez M, et al.*
摘要：研究重组HIST1H2AB的乙酰化修饰在干细胞分化过程中的动态变化，发现其修饰水平与特定分化基因的激活密切相关。
---
注：以上文献名为虚拟示例，实际研究需通过PubMed或Google Scholar检索真实文献。
The HIST1H2AB gene encodes a member of the histone H2A family, a core component of nucleosomes that organize eukaryotic DNA into chromatin. As part of the HIST1 cluster on chromosome 6. HIST1H2AB produces a replication-dependent histone critical for maintaining chromatin structure and regulating gene expression. Histone H2A, along with H2B, H3. and H4. forms the octameric core around which DNA wraps, influencing chromatin compaction and accessibility. Unlike canonical H2A variants, HIST1H2AB and other HIST1 cluster members are predominantly expressed during the S phase of the cell cycle to support DNA replication and cell division.
Recombinant HIST1H2AB protein is typically produced in bacterial or eukaryotic expression systems, enabling studies of its biochemical properties and post-translational modifications (PTMs). These modifications, such as phosphorylation, ubiquitination, and acetylation, play pivotal roles in DNA repair, transcriptional regulation, and epigenetic inheritance. Researchers utilize recombinant HIST1H2AB to investigate nucleosome assembly/disassembly dynamics, histone-DNA interactions, and the functional impact of specific PTMs using techniques like chromatin immunoprecipitation (ChIP) or in vitro reconstitution assays.
Additionally, this recombinant protein serves as an antigen for antibody development or a substrate for enzymes like histone acetyltransferases (HATs) or deubiquitinases. Its application extends to drug discovery, particularly in screening compounds targeting histone-modifying enzymes implicated in cancer or neurodegenerative diseases. Quality control involves mass spectrometry and electrophoretic analysis to ensure purity and proper folding. Studying HIST1H2AB contributes to understanding epigenetic mechanisms, chromatin remodeling, and disease-associated dysregulation of histone function.
×
