| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST2H2AA3 |
| Uniprot No | P04908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-130aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY SERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGR VTIAQGGVLP NIQAVLLPKK TESHHKAKGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST2H2AA3重组蛋白的模拟参考文献示例(注：部分文献为假设性概括，实际研究中请通过学术数据库核实)：
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1. **标题**: *"Recombinant expression and structural characterization of human HIST2H2AA3 in chromatin assembly"*
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了HIST2H2AA3重组蛋白，通过核磁共振(NMR)解析其三维结构，揭示了其在核小体组装中的关键作用，并验证了其与组蛋白H2B的相互作用模式。
2. **标题**: *"Post-translational modifications of HIST2H2AA3 regulate DNA damage response in vitro"*
**作者**: Lee S, et al.
**摘要**: 利用重组HIST2H2AA3蛋白，作者发现其C端磷酸化修饰(Ser122)可增强染色质松弛，促进DNA损伤修复蛋白BRCA1的招募，为组蛋白变体在基因组稳定性中的功能提供了新证据。
3. **标题**: *"High-yield production of recombinant HIST2H2AA3 for epigenetic studies"*
**作者**: Zhang Y, et al.
**摘要**: 开发了一种高效昆虫细胞表达系统，用于大规模生产HIST2H2AA3重组蛋白，并验证其在体外核小体重建实验中的功能性，为表观遗传学研究提供了可靠工具。
4. **标题**: *"HIST2H2AA3 interacts with HDAC1 to modulate gene silencing in cancer cells"*
**作者**: Tanaka K, et al.
**摘要**: 通过重组蛋白结合免疫共沉淀实验，发现HIST2H2AA3与组蛋白去乙酰化酶HDAC1存在直接相互作用，可能通过调控特定基因启动子区域的染色质压缩状态影响肿瘤进展。
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**备注**：以上文献为基于HIST2H2AA3功能假设的模拟结果。实际研究中建议通过PubMed、Web of Science等平台，结合关键词“HIST2H2AA3 recombinant protein”“H2A variant expression”等进行检索，并优先选择近5年内发表的文献以获取最新进展。
**Background of HIST2H2AA3 Recombinant Protein**
Histones are essential proteins involved in packaging DNA into nucleosomes, the fundamental units of chromatin. Among core histones, H2A is a key component of the nucleosome octamer, contributing to DNA stability and epigenetic regulation. The **HIST2H2AA3** gene, part of the histone H2A family located on chromosome 1q42.13 in humans, encodes a replication-dependent histone variant. This variant plays a role in maintaining chromatin structure during DNA replication and repair, as well as modulating transcriptional activity through post-translational modifications (e.g., acetylation, phosphorylation).
Recombinant HIST2H2AA3 protein is produced in vitro using expression systems such as *E. coli* or mammalian cells, enabling precise study of its biochemical and functional properties. Unlike native histones, recombinant versions are often tagged for purification (e.g., His-tag) and lack cell-specific modifications unless engineered, providing a standardized tool for research. Structural studies using recombinant HIST2H2AA3 help elucidate interactions with DNA and other histones (e.g., H2B, H3. H4) within nucleosomes. Its role in epigenetic mechanisms, such as marking sites for DNA damage response or regulating gene expression, is also explored in vitro.
Research applications include chromatin reconstitution assays, investigating histone exchange dynamics (e.g., with H2A.Z or macroH2A variants), and screening for histone-modifying enzyme inhibitors. Dysregulation of HIST2H2AA3 has been implicated in cancers and developmental disorders, making its recombinant form valuable for modeling disease-related chromatin alterations. Overall, HIST2H2AA3 recombinant protein serves as a critical reagent for advancing epigenetics, genome stability, and therapeutic discovery.
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