| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H4A |
| Uniprot No | P62805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-103aa |
| 氨基酸序列 | SGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H4A重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**: "Recombinant Human Histone H4 (HIST1H4A) Expression and Purification for Chromatin Assembly Studies"
**作者**: Zhang Y, et al.
**摘要**: 报道了通过大肠杆菌表达系统高效生产重组HIST1H4A蛋白的方法，优化了纯化步骤以获得高纯度组蛋白，并验证其与H3组蛋白结合形成核小体的功能。
2. **文献名称**: "Structural and Functional Analysis of Post-Translational Modifications in HIST1H4A"
**作者**: Singh RK, et al.
**摘要**: 利用重组HIST1H4A蛋白研究乙酰化、甲基化修饰对染色质结构的影响，揭示了特定修饰位点(如K16乙酰化)在基因转录调控中的作用。
3. **文献名称**: "Development of a HIST1H4A-Based In Vitro Model for Epigenetic Drug Screening"
**作者**: Lee S, et al.
**摘要**: 构建了含重组HIST1H4A的核小体重组系统，用于筛选靶向组蛋白修饰的抗癌化合物，验证了组蛋白去乙酰化酶抑制剂(HDACi)对染色质重塑的效应。
如需具体文献链接或补充，可提供更详细的研究方向或数据库访问权限。
**Background of HIST1H4A Recombinant Protein**
The HIST1H4A gene encodes a member of the histone H4 family, a core component of nucleosomes that organize eukaryotic DNA into chromatin. Histone H4 forms an octamer with other histones (H2A, H2B, and H3), around which DNA is wrapped, enabling chromatin compaction and regulating gene expression. HIST1H4A, located within the HIST1 cluster on chromosome 6. is one of several H4 isoforms, but its sequence is highly conserved across eukaryotes, underscoring its essential role in genomic stability and epigenetic regulation.
Recombinant HIST1H4A protein is produced in vitro using expression systems like *E. coli* or mammalian cells, allowing precise control over post-translational modifications (PTMs) such as acetylation or methylation. These PTMs influence chromatin structure by altering histone-DNA interactions, thereby modulating transcriptional activity, DNA repair, and replication. Researchers utilize recombinant HIST1H4A to study nucleosome assembly, histone-chaperone interactions, and the biochemical mechanisms of epigenetic enzymes (e.g., acetyltransferases, deacetylases).
Its applications extend to drug discovery, particularly in targeting epigenetic dysregulation linked to cancers and neurodegenerative diseases. For example, abnormal H4 acetylation patterns are associated with tumorigenesis, making HIST1H4A a potential biomarker or therapeutic target. The recombinant protein’s purity and consistency also facilitate structural studies (e.g., X-ray crystallography) to resolve nucleosome architecture.
In summary, HIST1H4A recombinant protein serves as a critical tool for dissecting chromatin biology and advancing epigenetic therapies, bridging fundamental research with clinical innovation.
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