| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AH |
| Uniprot No | Q96KK5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-128aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK TESHHKAK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H2AH重组蛋白的模拟参考文献示例(注：部分内容为假设性概括，实际文献需通过学术数据库查询)：
1. **文献名称**：*Expression and Purification of Recombinant HIST1H2AH in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效制备HIST1H2AH重组蛋白的方法，优化了表达条件及纯化步骤，并通过质谱验证其正确性，为后续功能研究提供材料基础。
2. **文献名称**：*HIST1H2AH Modulates Chromatin Accessibility in Gene Regulation*
**作者**：Zhang L, Wang Y.
**摘要**：通过体外染色质重构实验，发现HIST1H2AH重组蛋白参与调控核小体稳定性，影响特定基因启动子区域的染色质开放状态，提示其在表观遗传调控中的潜在作用。
3. **文献名称**：*Altered HIST1H2AH Expression in Breast Cancer Cell Lines*
**作者**：Johnson R, et al.
**摘要**：利用重组HIST1H2AH蛋白制备抗体，检测多种癌细胞系中该组蛋白变体的表达水平，发现其在乳腺癌细胞中显著下调，可能与肿瘤发生中的染色质异常相关。
4. **文献名称**：*Structural Insights into HIST1H2AH Interaction with Histone Chaperones*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析HIST1H2AH重组蛋白与伴侣蛋白DAXX的复合物结构，揭示其特异性结合界面，为理解组蛋白组装机制提供结构基础。
**提示**：以上为模拟示例，实际文献建议通过PubMed或Web of Science以关键词“HIST1H2AH recombinant”检索，或关注组蛋白修饰、染色质动力学相关领域的研究。
**Background of HIST1H2AH Recombinant Protein**
HIST1H2AH, a member of the histone H2A family, is a core component of nucleosomes, the fundamental units of eukaryotic chromatin. Histones play a critical role in DNA packaging, gene regulation, and epigenetic inheritance. The H2A family, including HIST1H2AH, contributes to nucleosome structure by forming a heterodimer with H2B, which interacts with H3-H4 tetramers to stabilize DNA wrapping.
As a replication-dependent histone, HIST1H2AH is encoded by the *HIST1H2AH* gene located within the large histone gene cluster on chromosome 6 (6p22.2). Its expression is tightly regulated during the cell cycle, peaking in S-phase to support DNA replication and chromatin assembly. Post-translational modifications (PTMs) of HIST1H2AH, such as phosphorylation, ubiquitination, or acetylation, influence chromatin dynamics, DNA repair, and transcriptional activity.
Recombinant HIST1H2AH protein is produced in vitro using expression systems like *E. coli* or mammalian cells, enabling controlled study of its biochemical and functional properties. The recombinant form is often engineered with tags (e.g., His, GST) for purification and detection. Researchers utilize this protein to investigate nucleosome remodeling, histone-DNA interactions, and the role of specific PTMs in diseases like cancer or developmental disorders.
Studies involving HIST1H2AH recombinant protein also contribute to understanding epigenetic mechanisms, such as how histone variants or mutations affect chromatin accessibility and gene expression. Its applications extend to drug discovery, particularly in targeting histone-modifying enzymes or chromatin-related pathways. Overall, HIST1H2AH serves as a vital tool for dissecting the molecular basis of genome organization and epigenetic regulation.
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