| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AG |
| Uniprot No | P0C0S8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-130aa |
| 氨基酸序列 | SGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK |
| 预测分子量 | 18.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于HIST1H2AG(组蛋白H2A家族成员)的研究相对细分，相关文献可能较少。以下是基于该领域常见研究方向的**模拟参考文献示例**，供参考：
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1. **文献名称**：*"Recombinant expression and functional analysis of human HIST1H2AG in nucleosome assembly"*
**作者**：Smith, J. et al.
**摘要**：研究报道了通过大肠杆菌系统重组表达HIST1H2AG蛋白的方法，并验证其与组蛋白H2B、H3和H4的相互作用，证明其在体外核小体重组中的关键作用。
2. **文献名称**：*"Epigenetic regulation mediated by HIST1H2AG phosphorylation: Insights from recombinant protein models"*
**作者**：Zhang, Y. et al.
**摘要**：利用重组HIST1H2AG蛋白研究其丝氨酸磷酸化修饰对染色质松解的影响，揭示了该修饰在DNA损伤修复通路中的潜在调控机制。
3. **文献名称**：*"Structural characterization of HIST1H2AG variants using recombinant protein crystallography"*
**作者**：Johnson, R. et al.
**摘要**：通过X射线晶体学解析重组HIST1H2AG蛋白的三维结构，探讨其C端结构域在核小体稳定性中的独特功能。
4. **文献名称**：*"HIST1H2AG overexpression in cancer: Recombinant protein-based biomarker discovery"*
**作者**：Lee, S. et al.
**摘要**：开发基于重组HIST1H2AG的抗体，分析其在多种肿瘤组织中的异常表达，提示其作为癌症诊断标志物的可能性。
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**说明**：
以上文献为模拟示例，实际研究中请通过**PubMed**、**Google Scholar**或**Web of Science**等平台，以关键词“HIST1H2AG recombinant protein”“H2A histone variants”等检索最新文献。若需具体文献协助，可提供更多背景信息进一步筛选。
The HIST1H2AG recombinant protein is derived from the HIST1H2AG gene, which encodes a member of the histone H2A family. Histones are essential nuclear proteins responsible for packaging DNA into structural units called nucleosomes, facilitating chromatin organization and regulating gene expression. The H2A family, including HIST1H2AG, forms the core histone octamer alongside H2B, H3. and H4. playing a critical role in maintaining genomic stability and epigenetic regulation. HIST1H2AG, specifically, is a replication-dependent histone expressed during the S phase of the cell cycle, contributing to chromatin assembly during DNA replication and repair.
Recombinant HIST1H2AG protein is produced using biotechnological methods, such as expression in bacterial or eukaryotic systems, followed by purification to ensure high homogeneity. This engineered protein retains functional properties of native histones, enabling its use in studying chromatin dynamics, nucleosome structure, and DNA-protein interactions. Researchers employ it to investigate post-translational modifications (e.g., acetylation, phosphorylation) that influence gene accessibility and cellular processes like transcription, replication, and damage response. Additionally, it serves as a tool for developing assays targeting epigenetic mechanisms or autoimmune diseases linked to anti-histone antibodies. Its recombinant nature allows controlled experimental conditions, bypassing variability inherent in isolating histones from native tissues. Studies leveraging HIST1H2AG recombinant protein contribute to advancing understanding of epigenetic dysregulation in cancer, neurodegeneration, and other pathologies.
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