| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST1H2AJ |
| Uniprot No | Q99878 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-128aa |
| 氨基酸序列 | SGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK TESHHKTK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST1H2AJ重组蛋白的3篇参考文献的示例(注：文献信息为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*"Recombinant HIST1H2AJ Protein as a Tool for Chromatin Dynamics Studies"*
**作者**：Chen L, et al.
**摘要**：本研究成功表达并纯化了重组HIST1H2AJ蛋白，验证其与DNA结合能力，并证明其在体外核小体重建实验中可增强染色质结构的稳定性，为表观遗传修饰研究提供了新工具。
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2. **文献名称**：*"Epigenetic Regulation of HIST1H2AJ in Breast Cancer Cells"*
**作者**：Wang Y, et al.
**摘要**：通过重组HIST1H2AJ蛋白的磷酸化突变体实验，发现其修饰异常与乳腺癌细胞的染色质异常开放状态相关，提示其在癌症表观遗传失调中的潜在作用。
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3. **文献名称**：*"Structural Insights into HIST1H2AJ-H2B Dimerization by Recombinant Protein Analysis"*
**作者**：Kimura S, et al.
**摘要**：利用重组HIST1H2AJ和H2B蛋白进行共结晶实验，解析了二者结合的分子机制，揭示了组蛋白H2A-H2B异源二聚体形成的关键结构域。
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**说明**：
- 上述文献为示例，实际文献需通过PubMed、Google Scholar等平台以关键词“HIST1H2AJ recombinant protein”或“HIST1H2AJ chromatin”检索。
- 若研究较少，可扩展至组蛋白H2A家族重组蛋白的通用功能或技术研究(如核小体重建、表观遗传学应用)。
HIST1H2AJ, a member of the histone H2A family, is a core component of nucleosomes, the fundamental repeating units of chromatin. As part of the HIST1 cluster on chromosome 6. it encodes a replication-dependent histone critical for DNA packaging and epigenetic regulation. This protein plays a role in maintaining chromatin structure, mediating DNA accessibility for transcription, replication, and repair. Unlike variant histones, HIST1H2AJ is predominantly expressed during the S phase of the cell cycle.
Recombinant HIST1H2AJ protein is produced through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells), often fused with tags like GST or His for purification. Its recombinant form enables controlled studies of histone interactions, post-translational modifications (e.g., acetylation, phosphorylation), and nucleosome assembly dynamics. Researchers utilize it to investigate epigenetic mechanisms, chromatin remodeling, and gene silencing/activation processes.
This protein is particularly valuable in structural studies (X-ray crystallography, cryo-EM) to resolve nucleosome organization and in biochemical assays assessing histone-DNA or histone-chaperone interactions. It also serves as a substrate for enzyme activity screens targeting histone-modifying proteins, with implications for cancer and developmental disorders. Recent studies explore its potential role in cellular differentiation and response to DNA damage. The availability of recombinant HIST1H2AJ accelerates mechanistic insights into chromatin biology and therapeutic targeting of epigenetic dysregulation.
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