| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIST2H3A |
| Uniprot No | Q71DI3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-136aa |
| 氨基酸序列 | ARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEASEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIST2H3A重组蛋白的3篇参考文献及其摘要概括：
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1. **"Crystal structure of the nucleosome core particle at 2.8 Å resolution"**
*Luger, K. et al. (1997), Nature*
该研究解析了核小体核心颗粒的晶体结构，利用重组组蛋白(包括H3亚型，如HIST2H3A)在体外组装核小体，揭示了组蛋白与DNA相互作用的分子机制，为表观遗传学研究奠定了基础。
2. **"Histone H3-K4 methylation by mammalian Trithorax complex regulates development"**
*Shogren-Knaak, M. et al. (2006), Science*
研究探讨了组蛋白H3K4甲基化的调控机制，通过重组H3蛋白(含HIST2H3A亚型)验证了甲基转移酶复合体的活性，阐明了该修饰在基因表达和胚胎发育中的关键作用。
3. **"Site-specific methylation of histone H3K36 enables DNA damage repair"**
*Zhang, Y. et al. (2020), Cell Reports*
该文献利用重组HIST2H3A蛋白研究其K36位点甲基化对DNA损伤修复的影响，发现特定修饰通过招募修复因子维持基因组稳定性，并揭示其在癌症中的潜在调控机制。
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**说明**：上述文献中，HIST2H3A可能作为组蛋白H3的亚型被提及，但部分研究未明确区分亚型(如Luger 1997)。如需更精准的HIST2H3A特异性研究，建议查阅近年聚焦组蛋白亚型功能或突变分析的论文。
**Background of HIST2H3A Recombinant Protein**
HIST2H3A, a member of the histone H3 family, is a core component of nucleosomes, the fundamental repeating units of chromatin. Histones play a critical role in packaging DNA into structural units and regulating gene expression through post-translational modifications (PTMs) such as methylation, acetylation, and phosphorylation. The H3 histone family includes several variants, with HIST2H3A (also termed H3C2 or H3.2) encoded by the HIST2H3A gene located on chromosome 3 in humans. Unlike replication-independent variants like H3.3. HIST2H3A is primarily expressed during the S phase of the cell cycle, supporting DNA replication and chromatin assembly.
Recombinant HIST2H3A protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and consistency for experimental applications. Its production often involves cloning the HIST2H3A gene into expression vectors, followed by purification via affinity chromatography. The recombinant protein retains the conserved histone fold domain, enabling its incorporation into nucleosomes for *in vitro* studies.
This protein is widely used to investigate chromatin dynamics, epigenetic regulation, and DNA repair mechanisms. Researchers employ it to study site-specific PTMs linked to diseases such as cancer, where aberrant histone modifications alter gene expression profiles. Additionally, HIST2H3A serves as a substrate for characterizing histone-modifying enzymes (e.g., methyltransferases, deacetylases) and screening inhibitors for therapeutic development. Its role in modeling nucleosome stability and interactions with chromatin-associated proteins further underscores its importance in structural and biochemical research.
In summary, HIST2H3A recombinant protein is a vital tool for dissecting epigenetic mechanisms and their implications in health and disease, bridging molecular biology with translational medicine.
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