| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H4 |
| Uniprot No | P62805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-103aa |
| 氨基酸序列 | SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLI YEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGF GG |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H4重组蛋白的3篇参考文献的简要列举(注：文献信息为虚构示例，仅供参考格式)：
1. **文献名称**：*Expression and Purification of Recombinant Histone H4 for Chromatin Assembly Studies*
**作者**：Smith A, et al.
**摘要**：研究报道了一种高效表达和纯化重组组蛋白H4的方法，验证其与DNA结合的能力，并应用于体外染色质重建实验。
2. **文献名称**：*Recombinant H4 Protein Modulates DNA Damage Response in Cancer Cells*
**作者**：Chen L, et al.
**摘要**：通过体外实验证明重组H4蛋白可增强癌细胞中DNA损伤修复信号通路活性，提示其在基因组稳定性中的潜在作用。
3. **文献名称**：*Structural Analysis of Recombinant H4-K16 Acetylation Using NMR Spectroscopy*
**作者**：Wang Y, et al.
**摘要**：利用核磁共振技术解析重组H4蛋白第16位赖氨酸乙酰化后的构象变化，揭示其对染色质高级结构的影响。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“recombinant H4 protein”或“histone H4 expression”获取具体研究。
**Background of H4 Recombinant Protein**
Histone H4 is a core component of nucleosomes, playing a critical role in chromatin structure and gene regulation. As one of the five major histone proteins (H2A, H2B, H3. H4. and linker histone H1), H4 binds to DNA to compact it into higher-order structures, ensuring genome stability and regulating transcription, replication, and repair. Its highly conserved N-terminal tail undergoes post-translational modifications (e.g., acetylation, methylation), which modulate chromatin accessibility and epigenetic signaling. Dysregulation of H4-related processes is linked to cancers, developmental disorders, and neurodegenerative diseases.
Recombinant H4 protein refers to the lab-engineered version produced via heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells). This approach allows large-scale, pure, and modification-specific production of H4. bypassing challenges in isolating it from native tissues. Researchers often tag recombinant H4 with markers (e.g., GFP, His-tags) for purification, tracking, or interaction studies.
The development of recombinant H4 has advanced studies in epigenetics and nucleosome dynamics. For instance, it enables *in vitro* reconstitution of nucleosomes to investigate how H4 modifications influence DNA-histone interactions or recruit chromatin remodelers. Additionally, recombinant H4 is used to screen drugs targeting histone-modifying enzymes (e.g., histone acetyltransferases) or to develop antibodies for diagnostic tools.
Despite its utility, producing functional recombinant H4 requires optimizing expression conditions to preserve post-translational modifications or mimic native folding. Ongoing innovations in protein engineering and synthetic biology aim to address these limitations, enhancing its application in basic research and therapeutic development.
In summary, recombinant H4 serves as a vital tool for dissecting chromatin biology and exploring epigenetic therapies, bridging molecular insights with clinical potential.
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