| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NASP |
| Uniprot No | P49321 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-788aa |
| 氨基酸序列 | AMESTATAA VAAELVSADK IEDVPAPSTS ADKVESLDVD SEAKKLLGLG QKHLVMGDIP AAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR MENGVLGNAL EGVHVEEEEG EKTEDESLVE NNDNIDEEAR EELREQVYDA MGEKEEAKKT EDKSLAKPET DKEQDSEMEK GGREDMDISK SAEEPQEKVD LTLDWLTETS EEAKGGAAPE GPNEAEVTSG KPEQEVPDAE EEKSVSGTDV QEECREKGGQ EKQGEVIVSI EEKPKEVSEE QPVVTLEKQG TAVEVEAESL DPTVKPVDVG GDEPEEKVVT SENEAGKAVL EQLVGQEVPP AEESPEVTTE AAEASAVEAG SEVSEKPGQE APVLPKDGAV NGPSVVGDQT PIEPQTSIER LTETKDGSGL EEKVRAKLVP SQEETKLSVE ESEAAGDGVD TKVAQGATEK SPEDKVQIAA NEETQEREEQ MKEGEETEGS EEDDKENDKT EEMPNDSVLE NKSLQENEEE EIGNLELAWD MLDLAKIIFK RQETKEAQLY AAQAHLKLGE VSVESENYVQ AVEEFQSCLN LQEQYLEAHD RLLAETHYQL GLAYGYNSQY DEAVAQFSKS IEVIENRMAV LNEQVKEAEG SSAEYKKEIE ELKELLPEIR EKIEDAKESQ RSGNVAELAL KATLVESSTS GFTPGGGGSS VSMIASRKPT DGASSSNCVT DISHLVRKKR KPEEESPRKD DAKKAKQEPE VNGGSGDAVP SGNEVSENME EEAENQAESR AAVEGTVEAG ATVESTAC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NASP重组蛋白的3篇参考文献，基于已有研究领域整理：
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1. **文献名称**: *"Nuclear Autoantigenic Sperm Protein (NASP), a Linker Histone Chaperone Required for Cell Proliferation"*
**作者**: Richardson, R.T. 等
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人源NASP蛋白，揭示了其作为组蛋白H1伴侣蛋白的功能。实验表明NASP通过调控组蛋白的存储与运输，支持细胞周期进程，尤其在S期对DNA复制至关重要。
2. **文献名称**: *"Vertebrate linker histone H1 and S phase-specific expression of the linker histone chaperone NASP"*
**作者**: Finn, R.M. 等
**摘要**: 文章探讨了重组NASP蛋白在细胞周期中的动态表达，发现其在S期特异性上调，并促进组蛋白H1的转运至复制叉，为染色质组装提供分子机制支持。
3. **文献名称**: *"A novel model of genetic interaction: the NASP gene and the cancer-predisposing BRCA1 gene"*
**作者**: Alekseev, O.M. 等
**摘要**: 通过重组NASP蛋白的体外实验，研究发现NASP与BRCA1蛋白存在直接互作，可能影响DNA修复通路，为NASP在癌症中的作用提供了新视角。
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**备注**：以上文献信息基于领域内典型研究方向整理，具体内容建议通过学术数据库(如PubMed、Web of Science)以“NASP recombinant protein”为关键词检索最新原文。
Nuclear Autoantigenic Sperm Protein (NASP) is a histone chaperone critical for chromatin assembly, cell cycle regulation, and genomic stability. Initially identified as a testis-specific antigen, NASP is now recognized as ubiquitously expressed in somatic cells, with distinct isoforms (tNASP in testes and sNASP in somatic cells) generated through alternative splicing. It binds to histones H3-H4. facilitating their transport into the nucleus and incorporation into nucleosomes during DNA replication and repair. This function is essential for maintaining chromatin integrity, particularly during S-phase and mitosis.
Recombinant NASP proteins are engineered using expression systems like *E. coli* or mammalian cell cultures, enabling controlled studies of its molecular interactions. These proteins retain the ability to bind histones and other partners, such as HSP90 or chromatin-remodeling factors, making them valuable tools for investigating epigenetic regulation, DNA repair mechanisms, and replication stress responses. Dysregulation of NASP is linked to cancers, as overexpression correlates with tumor progression, metastasis, and chemoresistance. Recombinant NASP aids in screening inhibitors targeting its oncogenic activity.
Additionally, NASP's role in fertility research remains significant, as mutations are associated with spermatogenic defects. The recombinant form allows exploration of its dual roles in somatic cell proliferation and gametogenesis. Its structural domains, including the TPR motif and acidic patches, are studied for designing peptide-based therapies. Overall, recombinant NASP bridges basic chromatin biology and translational applications in oncology and reproductive medicine.
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