| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDAC9 |
| Uniprot No | Q9UKV0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 548-1011aa |
| 氨基酸序列 | KEEPVDSDEDAQIQEMESGEQAAFMQQPFLEPTHTRALSVRQAPLAAVGM DGLEKHRLVSRTHSSPAASVLPHPAMDRPLQPGSATGIAYDPLMLKHQCV CGNSTTHPEHAGRIQSIWSRLQETGLLNKCERIQGRKASLEEIQLVHSEH HSLLYGTNPLDGQKLDPRILLGDDSQKFFSSLPCGGLGVDSDTIWNELHS SGAARMAVGCVIELASKVASGELKNGFAVVRPPGHHAEESTAMGFCFFNS VAITAKYLRDQLNISKILIVDLDVHHGNGTQQAFYADPSILYISLHRYDE GNFFPGSGAPNEVGTGLGEGYNINIAWTGGLDPPMGDVEYLEAFRTIVKP VAKEFDPDMVLVSAGFDALEGHTPPLGGYKVTAKCFGHLTKQLMTLADGR VVLALEGGHDLTAICDASEACVNALLGNELEPLAEDILHQSPNMNAVISL QKIIEIQSMSLKFS |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDAC9重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：*HDAC9 modulates endothelial cell proliferation and angiogenesis through histone deacetylase activity*
**作者**：Wang Y, et al.
**摘要**：研究利用重组HDAC9蛋白，揭示其通过去乙酰化组蛋白H3调控内皮细胞增殖和血管生成的分子机制。
2. **文献名称**：*Structural and functional characterization of recombinant HDAC9 catalytic domain*
**作者**：Smith JL, et al.
**摘要**：通过表达和纯化HDAC9重组催化结构域，分析其酶动力学特性及与小分子抑制剂的相互作用。
3. **文献名称**：*HDAC9 interacts with FOXP3 to regulate Treg cell function in autoimmune diseases*
**作者**：Li H, et al.
**摘要**：利用重组HDAC9蛋白进行体外结合实验，证明其与FOXP3的相互作用影响调节性T细胞的免疫抑制功能。
4. **文献名称**：*Recombinant HDAC9 as a therapeutic target in acute myeloid leukemia*
**作者**：Garcia-Manero G, et al.
**摘要**：探讨重组HDAC9蛋白在白血病细胞中的异常表达及其作为表观遗传治疗靶点的潜力。
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**建议**：您可通过PubMed或Google Scholar搜索关键词“recombinant HDAC9 protein”或“HDAC9 purification”获取最新及具体文献。经典研究可关注HDAC9在心血管疾病、癌症及免疫调控中的功能。
**Background of HDAC9 Recombinant Protein**
Histone deacetylase 9 (HDAC9) is a member of the class IIa HDAC family, which plays a critical role in epigenetic regulation by removing acetyl groups from histone lysine residues. This activity promotes chromatin condensation and transcriptional repression, influencing gene expression patterns essential for cellular differentiation, development, and homeostasis. HDAC9 is particularly notable for its tissue-specific roles, with high expression in the heart, brain, and skeletal muscle. It interacts with transcription factors like MEF2 to modulate pathways involved in cardiac morphogenesis, muscle function, and immune responses. Dysregulation of HDAC9 has been linked to diseases such as cancer, cardiovascular disorders, and neurological conditions, highlighting its therapeutic relevance.
Recombinant HDAC9 protein is produced using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to enable biochemical and functional studies. Purified HDAC9 retains deacetylase activity and is often fused with tags (e.g., GST, His) for simplified isolation and detection. Researchers utilize this protein to investigate enzymatic mechanisms, screen inhibitors, and explore protein-protein interactions. Structural studies of recombinant HDAC9 have provided insights into its regulatory domains, including the N-terminal nuclear localization signal and C-terminal catalytic domain, which exhibits lower intrinsic activity compared to other HDAC classes, suggesting reliance on co-factors for full functionality.
In disease contexts, HDAC9 overexpression correlates with tumor progression and poor prognosis in cancers like lymphoma and glioblastoma. Conversely, its suppression in atherosclerosis models reduces vascular inflammation, underscoring its dual roles in pathology. The development of HDAC9-targeted therapies, including selective inhibitors, remains an active area of research, though challenges like isoform specificity persist. Overall, recombinant HDAC9 serves as a vital tool for dissecting epigenetic mechanisms and advancing translational studies in precision medicine.
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