| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDAC6 |
| Uniprot No | Q9UBN7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1215aa |
| 氨基酸序列 | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELG LEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAV LDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVT HPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYI AWPLQGWQATFGGGDH PPKSDPMGHHHHHHGRRRASVAAGILVPRGSP GLDGIYARGIQMTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGA VPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLV LDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKE ELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASG SVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQ QKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKA SNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLV LVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLR ALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLV RSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNM MNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTC HSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRL VEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGH ALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASS QIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSA GFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISE SMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKV EDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGE ESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTS EEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGAT LAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQG TTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADS MLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTI QENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAY VHHQALLDVKNIAHQNKFGEDMPHPH |
| 预测分子量 | 161 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDAC6重组蛋白的3篇代表性文献(内容基于真实研究归纳，但标题与作者为虚拟示例)：
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1. **文献名称**：*Structural Basis of HDAC6 Ubiquitin-Binding Domain and Its Role in Autophagy Regulation*
**作者**：Zhang Y, et al.
**摘要**：通过X射线晶体学解析了人源HDAC6重组蛋白的泛素结合结构域(UBD)三维结构，揭示了其与泛素链特异性结合的分子机制，并证明UBD结构对HDAC6介导的自噬过程至关重要。
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2. **文献名称**：*Expression and Functional Characterization of Recombinant HDAC6 in Insect Cells*
**作者**：Smith JL, et al.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达并纯化HDAC6重组蛋白，证实其具有α-微管蛋白去乙酰化活性，且对选择性抑制剂Tubastatin A敏感，为药物筛选提供了可靠工具。
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3. **文献名称**：*HDAC6 Deacetylase Activity Assay Using a Fluorogenic Substrate: Kinetic Analysis and Inhibitor Screening*
**作者**：Wang H, et al.
**摘要**：开发基于荧光底物的HDAC6重组蛋白体外活性检测方法，系统比较其与其他HDAC亚型的底物特异性，并成功应用于小分子抑制剂的IC50值测定。
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**备注**：若需真实文献，建议通过PubMed或Web of Science搜索关键词“HDAC6 recombinant protein”，筛选高被引论文(如涉及HDAC6结构、酶活测定或药物开发的研究)。经典研究多发表于*Nature Structural & Molecular Biology*、*Journal of Biological Chemistry*等期刊。
**Background of HDAC6 Recombinant Protein**
Histone deacetylase 6 (HDAC6) is a unique member of the histone deacetylase family, primarily localized in the cytoplasm rather than the nucleus. Unlike other HDACs, which mainly target histones to regulate gene expression, HDAC6 predominantly modulates non-histone substrates, playing a critical role in cellular processes such as protein trafficking, cytoskeletal dynamics, and stress response. Structurally, HDAC6 contains two tandem catalytic deacetylase domains and a zinc-finger ubiquitin-binding domain, enabling its dual enzymatic activity and interaction with ubiquitinated proteins.
HDAC6 is best known for deacetylating α-tubulin, influencing microtubule stability and cell motility. It also regulates heat shock protein 90 (Hsp90) by deacetylation, thereby affecting client protein folding and degradation. Additionally, HDAC6 participates in aggresome formation, a mechanism for clearing misfolded proteins, linking it to neurodegenerative diseases like Alzheimer’s and Parkinson’s. Its role in immune response, viral infection, and cancer metastasis further underscores its biomedical relevance.
Recombinant HDAC6 protein, produced via heterologous expression systems (e.g., *E. coli* or insect cells), is a vital tool for studying HDAC6’s biochemical functions and screening inhibitors. Purified recombinant HDAC6 retains enzymatic activity, enabling *in vitro* assays to explore substrate specificity, kinetic properties, and inhibitor efficacy. Its use has accelerated drug discovery, particularly for HDAC6-selective inhibitors, which are investigated as potential therapeutics for cancer, inflammation, and neurodegeneration with fewer side effects compared to pan-HDAC inhibitors.
Overall, HDAC6 recombinant protein serves as a cornerstone for deciphering the enzyme’s biological roles and developing targeted therapies.
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