| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUP85 |
| Uniprot No | Q9BW27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656aa |
| 氨基酸序列 | MEELDGEPTVTLIPGVNSKKNQMYFDWGPGEMLVCETSFNKKEKSEMVPSCPFIYIIRKDVDVYSQILRKLFNESHGIFLGLQRIDEELTGKSRKSQLVRVSKNYRSVIRACMEEMHQVAIAAKDPANGRQFSSQVSILSAMELIWNLCEILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGSENPSKHDSFWNLVTILVLQGRLDEARQMLSKEADASPASAGICRIMGDLMRTMPILSPGNTQTLTELELKWQHWHEECERYLQDSTFATSPHLESLLKIMLGDEAALLEQKELLSNWYHFLVTRLLYSNPTVKPIDLHYYAQSSLDLFLGGESSPEPLDNILLAAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCKLLQSHNLYFGSNMREFLLLEYASGLFAHPSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKALKVLRICEQRQMTEQVRSICKILAMKAVRNNRLGSALSWSIRAKDAAFATLVSDRFLRDYCERGCFSDLDLIDNLGPAMMLSDRLTFLGKYREFHRMYGEKRFADAASLLLSLMTSRIAPRSFWMTLLTDALPLLEQKQVIFSAEQTYELMRCLEDLTSRRPVHGESDTEQLQDDDIETTKVEMLRLSLARNLARAIIREGSLEGS |
| 预测分子量 | 82.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUP85重组蛋白的3-4篇参考文献的示例(文献信息为示例性概括，具体文献需通过学术数据库核实)：
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1. **文献名称**: *Structural insights into the Nup84 complex assembly in nuclear pore biogenesis*
**作者**: Hoelz A., et al.
**摘要**: 该研究通过重组表达NUP85及其互作蛋白(如NUP133、NUP160)，解析了酵母核孔复合体(NPC)中Nup84亚复合体的组装机制，揭示了NUP85在维持核孔结构完整性和物质运输中的关键作用。
2. **文献名称**: *NUP85 depletion disrupts the nuclear pore complex and induces p53-dependent senescence in colorectal cancer cells*
**作者**: Zhang Y., et al.
**摘要**: 利用重组NUP85蛋白进行功能回补实验，发现NUP85缺失导致核孔复合体异常，激活p53通路并诱导癌细胞衰老，提示其在肿瘤发生中的潜在治疗靶点价值。
3. **文献名称**: *Recombinant expression and interaction mapping of NUP85 reveals its role in mRNA export*
**作者**: Almazni I., et al.
**摘要**: 通过大肠杆菌系统重组表达人源NUP85蛋白，结合免疫共沉淀技术鉴定了其与NUP107、NUP133等核孔蛋白的相互作用网络，并验证了其在mRNA出核运输中的功能。
4. **文献名称**: *Mutation analysis of NUP85 in inherited renal diseases and functional validation via recombinant models*
**作者**: Braun DA., et al.
**摘要**: 研究报道了NUP85基因突变与遗传性肾病的关联，通过重组蛋白模型证实突变导致核孔复合体组装缺陷，影响细胞核质运输功能。
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**注意**：以上文献为示例，实际文献需通过**PubMed/Google Scholar**等平台以关键词“NUP85 recombinant”“NUP85 structure/function”检索。如需具体文献，建议补充研究领域或应用方向以进一步筛选。
**Background of NUP85 Recombinant Protein**
NUP85 (Nucleoporin 85) is a critical component of the nuclear pore complex (NPC), a large protein assembly embedded in the nuclear envelope that regulates nucleocytoplasmic transport of macromolecules, including RNAs and proteins. As part of the Nup107-160 subcomplex, NUP85 contributes to the structural scaffold of the NPC, ensuring its stability and mediating interactions with transport factors. This subcomplex is essential for proper NPC assembly, mitotic progression, and chromatin organization.
The recombinant NUP85 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the protein for research. Recombinant NUP85 enables detailed studies of its role in nuclear transport, NPC architecture, and interactions with other nucleoporins or regulatory molecules. Dysregulation of NUP85 has been linked to diseases such as cancer and developmental disorders, highlighting its biological significance.
Studies using recombinant NUP85 have advanced understanding of its structural domains, including its β-propeller and α-solenoid folds, which facilitate protein-protein interactions within the NPC. Additionally, it serves as a tool to explore mutations or post-translational modifications affecting NPC function. Research on NUP85 also intersects with virology, as some viruses exploit NPC components for nuclear entry.
Overall, recombinant NUP85 is pivotal for dissecting NPC mechanics, cellular homeostasis, and disease mechanisms, offering potential therapeutic insights for NPC-related pathologies.
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