| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MUC4 |
| Uniprot No | Q99102 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MUC4重组蛋白相关研究的文献示例(注：内容为概括性描述，具体文献信息需通过学术数据库验证)：
1. **《Recombinant MUC4 glycoprotein: a tool for studying HER2 signaling in cancer》**
- **作者**: Carraway KL, et al.
- **摘要**: 研究利用重组MUC4蛋白揭示其与HER2受体相互作用，证实MUC4通过激活HER2促进肿瘤细胞迁移和侵袭，为靶向治疗提供依据。
2. **《Expression and purification of the extracellular domain of human MUC4 mucin in Escherichia coli》**
- **作者**: Monzavi-Karbassi B, et al.
- **摘要**: 描述在大肠杆菌中表达MUC4胞外域重组蛋白的技术方法，优化纯化流程，验证其抗原性用于癌症免疫研究。
3. **《MUC4 mucin- a potential biomarker for pancreatic cancer detection》**
- **作者**: Chaturvedi P, et al.
- **摘要**: 分析重组MUC4蛋白在胰腺癌患者血清中的表达水平，提出其作为早期诊断标志物的潜力，并探讨其与化疗耐药性的关联。
**提示**：建议通过PubMed或Google Scholar检索最新文献，关键词包括"MUC4 recombinant protein"、"MUC4 expression"等，以获取结构解析、功能验证或临床应用的最新进展。
Mucin 4 (MUC4), a high-molecular-weight transmembrane glycoprotein, belongs to the mucin family characterized by heavily glycosylated tandem repeat domains. It plays critical roles in epithelial lubrication, cell signaling, and protection against pathogens. Structurally, MUC4 consists of a large extracellular α-subunit (carrying mucin-type O-glycans) non-covalently linked to a β-subunit containing epidermal growth factor (EGF)-like domains, a transmembrane region, and a cytoplasmic tail. This unique structure enables MUC4 to participate in cellular adhesion, differentiation, and oncogenic signaling pathways.
Recombinant MUC4 proteins are engineered through molecular cloning techniques, typically expressed in mammalian (e.g., CHO, HEK293) or insect cell systems to ensure proper post-translational modifications. These recombinant versions allow researchers to study MUC4's functional domains without interference from its natural complexity. Key applications include investigating its interaction with receptor tyrosine kinases like ErbB2. which promotes cancer cell proliferation and metastasis, particularly in pancreatic, ovarian, and lung cancers where MUC4 is overexpressed.
Current research focuses on MUC4's dual role as a tumor biomarker and therapeutic target. Recombinant proteins facilitate the development of diagnostic antibodies and vaccines, while also serving as tools to decipher mechanisms underlying chemoresistance and immune evasion in mucin-rich tumors. Challenges remain in replicating its native glycosylation patterns, which significantly influence biological interactions. Ongoing optimization of expression systems aims to enhance the clinical relevance of recombinant MUC4 for both basic research and translational applications.
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