| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCHR1 |
| Uniprot No | Q99705 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-353aa |
| 氨基酸序列 | MDLEASLLPTGPNASNTSDGPDNLTSAGSPPRTGSISYINIIMPSVFGTICLLGIIGNSTVIFAVVKKSKLHWCNNVPDIFIINLSVVDLLFLLGMPFMIHQLMGNGVWHFGETMCTLITAMDANSQFTSTYILTAMAIDRYLATVHPISSTKFRKPSVATLVICLLWALSFISITPVWLYARLIPFPGGAVGCGIRLPNPDTDLYWFTLYQFFLAFALPFVVITAAYVRILQRMTSSVAPASQRSIRLRTKRVTRTAIAICLVFFVCWAPYYVLQLTQLSISRPTLTFVYLYNAAISLGYANSCLNPFVYIVLCETFRKRLVLSVKPAAQGQLRAVSNAQTADEERTESKGT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCHR1重组蛋白的参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant Melanin-Concentrating Hormone Receptor 1 (MCHR1) in Mammalian Cells"
**作者**: Smith A, et al.
**摘要**: 本研究利用HEK293细胞系统成功表达并纯化MCHR1重组蛋白，证实其与MCH结合后激活G蛋白信号通路的能力，为体外药物筛选提供了模型。
2. **文献名称**: "Structural Insights into MCHR1 Activation Revealed by Cryo-EM"
**作者**: Zhang L, et al.
**摘要**: 通过冷冻电镜解析了MCHR1与其激动剂复合物的三维结构，揭示了受体跨膜区的构象变化及配体结合的关键氨基酸残基。
3. **文献名称**: "Development of a Radioligand Binding Assay for MCHR1 Using Recombinant Protein"
**作者**: Tanaka K, et al.
**摘要**: 建立了基于MCHR1重组蛋白的放射性配体结合实验，筛选出多个小分子拮抗剂，为肥胖相关疾病的药物研发提供工具。
4. **文献名称**: "MCHR1-Mediated Energy Homeostasis Studied via Recombinant Receptor Transgenic Models"
**作者**: Johnson R, et al.
**摘要**: 利用转基因小鼠模型证明MCHR1重组蛋白在能量代谢中的调控作用，发现其缺失导致摄食减少和能量消耗增加。
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注：以上文献为示例，实际引用需查询真实数据库(如PubMed)。
Melanin-concentrating hormone receptor 1 (MCHR1) is a G protein-coupled receptor (GPCR) that plays a critical role in regulating energy homeostasis, feeding behavior, and emotional responses. It is primarily activated by melanin-concentrating hormone (MCH), a neuropeptide produced in the hypothalamus. MCHR1 is widely expressed in the brain, particularly in regions associated with appetite control, reward pathways, and stress regulation, such as the hippocampus, amygdala, and nucleus accumbens. Its signaling involves coupling to Gαi/o proteins, leading to inhibition of cAMP production and activation of intracellular pathways like MAPK/ERK.
Research has linked MCHR1 dysfunction to metabolic and neuropsychiatric disorders, including obesity, depression, and anxiety. Pharmacological studies targeting MCHR1 have explored its potential as a therapeutic target for anti-obesity drugs, though early clinical trials faced challenges due to off-target effects or insufficient efficacy. Recombinant MCHR1 proteins, engineered through heterologous expression systems (e.g., mammalian, insect, or bacterial cells), are essential tools for structural studies, ligand binding assays, and drug discovery. These proteins often incorporate tags (e.g., His-tag) for purification and stabilization in membrane-mimetic environments. Recent advancements in cryo-EM and X-ray crystallography using recombinant MCHR1 have provided insights into its activation mechanisms and interaction with antagonists, aiding the design of selective modulators. Ongoing research continues to unravel its complex physiology and therapeutic potential in metabolic and CNS disorders.
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