| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | MIA1 |
| Uniprot No | Q61865 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-130aa |
| 氨基酸序列 | DRAMPKLA DWKLCADEEC SHPISMAVAL QDYVAPDCRF LTIYRGQVVY VFSKLKGRGR LFWGGSVQGG YYGDLAARLG YFPSSIVRED LTLKPGKIDM KTDQWDFYCQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MIA1重组蛋白的3篇参考文献(文献标题及摘要为模拟概括，非真实文献)：
1. **标题**：*Recombinant MIA1 Protein Inhibits Melanoma Cell Invasion In Vitro*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化重组MIA1蛋白，发现其通过结合细胞表面整合素抑制黑色素瘤细胞的迁移和侵袭能力，提示其作为潜在抗转移治疗分子。
2. **标题**：*Structural Characterization and Functional Analysis of MIA1 in Tumor Extracellular Matrix Remodeling*
**作者**：Zhang L, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度MIA1重组蛋白，通过晶体结构解析和体外实验揭示其通过SH3结构域与胞外基质蛋白相互作用，调控肿瘤微环境重塑。
3. **标题**：*MIA1 Recombinant Protein Attenuates Cartilage Degeneration in Osteoarthritis Models*
**作者**：Chen R, et al.
**摘要**：通过哺乳动物细胞表达MIA1重组蛋白，证明其可抑制软骨细胞凋亡和炎症因子释放，在骨关节炎小鼠模型中显著减缓软骨退变进程。
(注：以上内容为基于领域知识的模拟生成，实际文献需通过PubMed/Google Scholar等数据库以关键词“MIA1 recombinant protein”检索验证。)
**Background of MIA1 Recombinant Protein**
MIA1 (Melanoma Inhibitory Activity 1), also known as CD-RAP (Cartilage-Derived Retinoic Acid-Sensitive Protein), is a secreted protein initially identified in melanoma cells for its role in suppressing tumor progression and metastasis. Structurally, it belongs to the MIA/OTOR family, characterized by a conserved SH3 domain that facilitates interactions with extracellular matrix components and cell surface receptors. MIA1 is encoded by the *MIA1* gene and is physiologically expressed in cartilage, neural tissues, and certain epithelial cells, playing roles in cell adhesion, differentiation, and tissue remodeling.
Recombinant MIA1 protein is produced using genetic engineering techniques, often in bacterial or mammalian expression systems, to ensure high purity and bioactivity. Its study has gained momentum due to its dual role in cancer biology. While early studies highlighted its tumor-suppressive properties in melanoma by inhibiting cell migration and invasion, recent research reveals context-dependent oncogenic functions. For instance, MIA1 overexpression in breast, pancreatic, or colorectal cancers correlates with enhanced metastasis, chemoresistance, and poor prognosis, likely mediated through integrin-mediated signaling or modulation of MAPK/ERK pathways.
Beyond oncology, MIA1 is implicated in cartilage development and degenerative diseases. It regulates chondrocyte differentiation and matrix production, making it a potential biomarker for osteoarthritis or cartilage repair therapies. Additionally, its involvement in neuronal survival and axon guidance suggests relevance in neuroregeneration.
The development of recombinant MIA1 has enabled mechanistic studies, antibody production, and drug screening. However, conflicting data on its pro- and anti-tumor effects underscore the need for tissue- and context-specific investigations. Current research focuses on deciphering its molecular interactors, such as fibronectin or α4β1 integrin, and exploring therapeutic strategies targeting MIA1 in precision medicine. Overall, MIA1 recombinant protein serves as a critical tool for unraveling its multifaceted roles in health and disease.
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