纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | TAAR2 |
Uniprot No | Q9P1P5 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-351aa |
氨基酸序列 | MAVSSEQHELSHFKRTQTKKEKFNCSEYGNRSCPENERSLGVRVAMYSFMAGSIFITIFGNLAMIISISYFKQLHTPTNFLILSMAITDFLLGFTIMPYSMIRSVENCWYFGLTFCKIYYSFDLMLSITSIFHLCSVAIDRFYAICYPLLYSTKITIPVIKRLLLLCWSVPGAFAFGVVFSEAYADGIEGYDILVACSSSCPVMFNKLWGTTLFMAGFFTPGSMMVGIYGKIFAVSRKHAHAINNLRENQNNQVKKDKKAAKTLGIVIGVFLLCWFPCFFTILLDPFLNFSTPVVLFDALTWFGYFNSTCNPLIYGFFYPWFRRALKYILLGKIFSSCFHNTILCMQKESE |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAAR2重组蛋白的3篇代表性文献示例(注:文献内容为模拟概括,具体引用请核实真实数据库):
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1. **文献名称**: "Expression and Functional Characterization of Recombinant TAAR2 in Mammalian Cells"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌和HEK293细胞中表达了TAAR2重组蛋白,并验证了其膜定位及G蛋白偶联活性。通过荧光标记和cAMP检测,发现TAAR2对痕量胺类化合物(如苯乙胺)具有剂量依赖性响应,提示其在神经信号传导中的潜在作用。
2. **文献名称**: "Structural Insights into TAAR2 Ligand Binding through Cryo-EM Analysis"
**作者**: Zhang L, et al.
**摘要**: 利用冷冻电镜技术解析了TAAR2重组蛋白与其天然配体的复合物结构,揭示了配体结合口袋的关键氨基酸残基。研究为设计TAAR2特异性激动剂/拮抗剂提供了结构基础,并探讨了TAAR2在嗅觉感知中的非经典功能。
3. **文献名称**: "TAAR2 Knockout Mice and Recombinant Protein Rescue Experiments"
**作者**: Tanaka K, et al.
**摘要**: 通过构建TAAR2基因敲除小鼠模型,发现其嗅觉辨别能力受损。体外重组TAAR2蛋白回补实验证实,TAAR2重组蛋白可部分恢复嗅觉神经元对特定胺类分子的敏感性,支持其在化学感应通路中的功能。
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**注意**:以上文献信息为模拟生成,实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词:TAAR2. recombinant protein, trace amine receptor)。TAAR2研究相对较少,可扩展至TAAR家族其他成员(如TAAR1)的文献参考。
TAAR2 (Trace Amine-Associated Receptor 2) is a member of the trace amine-associated receptor (TAAR) family, a class of G protein-coupled receptors (GPCRs) that evolved to detect endogenous trace amines—biogenic amines like tyramine, β-phenylethylamine, and octopamine, which are structurally similar to classical neurotransmitters but present at lower concentrations. TAAR2 is primarily expressed in the central nervous system, with emerging evidence linking it to neuropsychiatric disorders such as schizophrenia, depression, and bipolar disorder. Unlike TAAR1. which has been extensively studied for its role in modulating dopamine and serotonin systems, TAAR2’s physiological functions remain less understood, partly due to its low endogenous expression and species-specific differences in ligand recognition.
Recombinant TAAR2 protein is engineered for in vitro studies to overcome challenges in isolating native receptors. It is typically produced by cloning the TAAR2 gene into expression vectors (e.g., bacterial, insect, or mammalian systems), followed by transfection, protein purification via affinity chromatography, and functional validation. This approach enables researchers to study TAAR2’s ligand-binding properties, signaling pathways (e.g., cAMP modulation), and interactions with potential therapeutics. Recent studies suggest TAAR2 may influence behaviors related to stress response and cognitive function, making it a tentative target for neuropharmacology. However, its orphan receptor status (lack of confirmed endogenous ligands in humans) complicates mechanistic studies. Recombinant TAAR2 tools are critical for high-throughput drug screening and structural studies (e.g., cryo-EM) to elucidate activation mechanisms and design selective agonists/antagonists, potentially advancing treatments for neurological and metabolic disorders.
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