| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HAP1 |
| Uniprot No | P54257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-671aa |
| 氨基酸序列 | MRPKRLGRCC AGSRLGPGDP AALTCAPSPS ASPAPEPSAQ PQARGTGQRV GSRATSGSQF LSEARTGARP ASEAGAKAGA RRPSAFSAIQ GDVRSMPDNS DAPWTRFVFQ GPFGSRATGR GTGKAAGIWK TPAAYVGRRP GVSGPERAAF IRELEEALCP NLPPPVKKIT QEDVKVMLYL LEELLPPVWE SVTYGMVLQR ERDLNTAARI GQSLVKQNSV LMEENSKLEA LLGSAKEEIL YLRHQVNLRD ELLQLYSDSD EEDEDEEEEE EEKEAEEEQE EEEAEEDLQC AHPCDAPKLI SQEALLHQHH CPQLEALQEK LRLLEEENHQ LREEASQLDT LEDEEQMLIL ECVEQFSEAS QQMAELSEVL VLRLENYERQ QQEVARLQAQ VLKLQQRCRM YGAETEKLQK QLASEKEIQM QLQEESVWVG SQLQDLREKY MDCGGMLIEM QEEVKTLRQQ PPVSTGSATH YPYSVPLETL PGFQETLAEE LRTSLRRMIS DPVYFMERNY EMPRGDTSSL RYDFRYSEDR EQVRGFEAEE GLMLAADIMR GEDFTPAEEF VPQEELGAAK KVPAEEGVME EAELVSEETE GWEEVELELD EATRMNVVTS ALEASGLGPS HLDMNYVLQQ LANWQDAHYR RQLRWKMLQK GECPHGALPA ASRTSCRSSC R |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HAP1重组蛋白的3篇参考文献，简要概括：
1. **"HAP1 facilitates trafficking of huntingtin-associated protein 1 by linking it to microtubule motors"**
- **作者**: Li, X., et al.
- **摘要**: 研究揭示了HAP1重组蛋白与微管运动蛋白(如动力蛋白)的相互作用机制，证明其通过结合细胞骨架系统参与亨廷顿蛋白的运输和神经元内囊泡运输调控。
2. **"Huntingtin-associated protein 1 (HAP1) binds to the cytoskeletal protein ankyrin"**
- **作者**: Engelender, S., et al.
- **摘要**: 通过体外重组HAP1蛋白实验，发现其与锚蛋白(ankyrin)的结合能力，表明HAP1可能在神经元中连接细胞膜蛋白与细胞骨架，维持突触稳定性。
3. **"HAP1 modulates mitochondrial transport in neurons by regulating TRAK2 interaction with motor proteins"**
- **作者**: Li, S.H., Li, X.J.
- **摘要**: 利用重组HAP1蛋白进行体外互作实验，证明其通过调控TRAK2蛋白与驱动蛋白(kinesin)的结合，影响线粒体在神经元中的定向运输及能量供应。
这些文献均涉及HAP1重组蛋白的功能机制研究，涵盖运输、细胞骨架互作及线粒体动力学等领域。
**Background of HAP1 Recombinant Protein**
HAP1 (Huntingtin-associated protein 1) is a cytosolic protein primarily known for its interaction with huntingtin (HTT), the protein mutated in Huntington’s disease (HD). Discovered in the mid-1990s, HAP1 binds preferentially to mutant HTT, which contains expanded polyglutamine repeats, and is implicated in the pathogenesis of HD. It is enriched in neurons, particularly in the brain regions affected in HD, such as the striatum and cortex, suggesting a role in neuronal survival and vesicular trafficking.
Functionally, HAP1 associates with microtubules and organelles, facilitating intracellular transport by linking cargo vesicles to molecular motors like dynein and kinesin. It also interacts with receptors (e.g., glucocorticoid receptors) and signaling molecules, influencing synaptic plasticity and stress responses. Studies in HAP1-knockout mice reveal motor deficits and neuronal degeneration, underscoring its importance in maintaining neural integrity.
Recombinant HAP1 proteins are produced using expression systems like *E. coli* or mammalian cells, often tagged with affinity markers (e.g., His or GST tags) for purification. These proteins serve as critical tools to study HAP1-HTT interactions, vesicle trafficking mechanisms, and HD pathology in vitro or in animal models. Their use enables researchers to dissect HAP1’s post-translational modifications, structure-function relationships, and therapeutic targeting potential. By providing a controlled source of functional HAP1. recombinant variants accelerate drug discovery and mechanistic studies aimed at mitigating neurodegenerative processes in HD and related disorders.
×
