| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SDHB |
| Uniprot No | P21912 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-280aa |
| 氨基酸序列 | AQ TAAATAPRIK KFAIYRWDPD KAGDKPHMQT YEVDLNKCGP MVLDALIKIK NEVDSTLTFR RSCREGICGS CAMNINGGNT LACTRRIDTN LNKVSKIYPL PHMYVIKDLV PDLSNFYAQY KSIEPYLKKK DESQEGKQQY LQSIEEREKL DGLYECILCA CCSTSCPSYW WNGDKYLGPA VLMQAYRWMI DSRDDFTEER LAKLQDPFSL YRCHTIMNCT RTCPKGLNPG KAIAEIKKMM ATYKEKKASV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SDHB重组蛋白的参考文献示例(注：内容为示例性质，非真实文献)：
1. **文献名称**：*Heterologous Expression and Functional Characterization of Recombinant SDHB in Mitochondrial Complex II*
**作者**：Zhang Y, et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化了重组人源SDHB蛋白，验证其与SDHA亚基的相互作用，并证明其在线粒体复合体II电子传递链中的关键作用，为解析SDHB突变致病机制提供基础。
2. **文献名称**：*Impact of SDHB Mutations on Succinate Dehydrogenase Activity Using Recombinant Protein Models*
**作者**：Gimenez-Roqueplo AP, et al.
**摘要**：通过构建携带家族性副神经节瘤相关SDHB突变的重组蛋白，发现特定突变(如p.Arg230His)显著降低酶活性，揭示了突变导致复合体II功能缺陷的分子机制。
3. **文献名称**：*Structural Insights into SDHB Deficiency Syndrome via Cryo-EM Analysis of Recombinant Complex II*
**作者**：Fukuoka M, et al.
**摘要**：采用冷冻电镜解析了重组SDHB与复合体II其他亚基的组装结构，发现SDHB C端结构域对复合体稳定性至关重要，为靶向治疗SDHB缺陷相关癌症提供依据。
4. **文献名称**：*Development of a Yeast Model for SDHB-Associated Metabolic Dysfunction*
**作者**：Bardella C, et al.
**摘要**：在酵母中异源表达人源SDHB重组蛋白，模拟致病突变导致的琥珀酸积累和氧化应激，证实SDHB失活与肿瘤微环境代谢重塑的关联。
(提示：实际研究中建议通过PubMed或Web of Science以“SDHB recombinant protein”为关键词检索最新文献。)
**Background of SDHB Recombinant Protein**
Succinate dehydrogenase complex subunit B (SDHB) is a critical component of mitochondrial complex II (succinate dehydrogenase), which plays a dual role in the tricarboxylic acid (TCA) cycle and the electron transport chain. It catalyzes the oxidation of succinate to fumarate, linking energy production pathways to cellular respiration. SDHB’s function is vital for maintaining metabolic homeostasis, and mutations in the *SDHB* gene are strongly associated with hereditary cancer syndromes, including paragangliomas, pheochromocytomas, and renal cell carcinomas. These mutations disrupt cellular energy metabolism and promote tumorigenesis through pseudohypoxic signaling and epigenetic dysregulation.
Recombinant SDHB protein is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems, to study its structural, functional, and biochemical properties. This engineered protein retains the native structure and enzymatic activity of wild-type SDHB, enabling researchers to investigate its role in mitochondrial dysfunction, metabolic reprogramming in cancer, and oxidative stress responses.
Applications of SDHB recombinant protein span disease mechanism studies, diagnostic tool development (e.g., antibody validation), and drug screening for therapies targeting SDH-deficient cancers. Its use has advanced understanding of how SDHB loss destabilizes complex II, drives succinate accumulation, and activates oncogenic pathways like HIF-1α. Additionally, recombinant SDHB serves as a reference standard in clinical assays to detect pathogenic variants or assess protein expression in tumor samples.
Overall, SDHB recombinant protein is a pivotal tool in mitochondrial biology and oncology research, offering insights into metabolic diseases and precision medicine strategies for SDH-related malignancies.
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