| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALOX12 |
| Uniprot No | P18054 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-663aa |
| 氨基酸序列 | MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPLVMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI |
| 预测分子量 | 79.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALOX12重组蛋白的3篇参考文献摘要概览：
---
1. **文献名称**：*"Purification and characterization of recombinant human platelet 12-lipoxygenase expressed in insect cells"*
**作者**：H. Kühn et al. (1993)
**摘要**：研究报道了利用杆状病毒-昆虫细胞系统成功表达并纯化重组人血小板12-脂氧合酶(ALOX12)，证实其酶活性与天然蛋白一致，为后续功能研究提供了可靠工具。
---
2. **文献名称**：*"12-Lipoxygenase inhibition sensitizes colorectal cancer cells to radiation treatment by suppressing cancer stem cell phenotype"*
**作者**：Y. Chen et al. (2019)
**摘要**：通过抑制ALOX12活性，研究证明其可降低结直肠癌干性标志物表达，增强癌细胞对放疗的敏感性，提示ALOX12作为潜在癌症治疗靶点。
---
3. **文献名称**：*"Crystal structure of a lipoxygenase from Cyanothece sp. reveals a novel helical dimerization motif"*
**作者**：S.A. Gillmor et al. (2017)
**摘要**：解析了蓝藻来源的12-脂氧合酶同源重组蛋白的晶体结构，揭示了独特的螺旋二聚化模式，为理解ALOX12家族催化机制提供结构基础。
---
4. **文献名称**：*"Recombinant 12-lipoxygenase catalytic domain properties and inhibition by a novel synthetic compound"*
**作者**：M. Rakonjac et al. (2016)
**摘要**：表达并表征了人ALOX12催化结构域重组蛋白，筛选出小分子抑制剂，证实其通过阻断底物通道降低酶活性，推动抗炎药物开发。
---
以上研究涵盖ALOX12重组蛋白的表达纯化、结构解析、功能机制及治疗应用，为相关领域提供关键参考。
ALOX12 (Arachidonate 12-Lipoxygenase) is a member of the lipoxygenase enzyme family, which catalyzes the oxidation of polyunsaturated fatty acids, particularly arachidonic acid, to produce bioactive lipid mediators. Specifically, ALOX12 introduces molecular oxygen into arachidonic acid at the 12th carbon position, generating 12-hydroperoxyeicosatetraenoic acid (12-HPETE), which is subsequently reduced to 12-hydroxyeicosatetraenoic acid (12-HETE). These metabolites play critical roles in cellular signaling, inflammation, and disease pathways, including cancer progression, cardiovascular disorders, and metabolic syndromes.
The recombinant ALOX12 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*), insect, or mammalian cell systems to ensure proper folding and enzymatic activity. Recombinant production allows for high-purity, scalable yields, enabling detailed biochemical studies and drug discovery efforts. Researchers utilize this protein to investigate substrate specificity, catalytic mechanisms, and interactions with inhibitors or modulators. Its structure-function relationships, including the role of conserved histidine residues and iron-binding motifs in catalysis, are key research focuses.
ALOX12 has garnered attention for its dual roles in health and disease. While it contributes to normal physiological processes like platelet activation and skin differentiation, dysregulation is linked to pathological conditions. For example, elevated 12-HETE levels are associated with tumor angiogenesis and metastasis in certain cancers. Recombinant ALOX12 facilitates the development of targeted therapies, such as small-molecule inhibitors, to modulate its activity in disease contexts. Additionally, genetic variants of ALOX12 are studied for their associations with diabetes, Alzheimer’s, and atherosclerosis, highlighting its therapeutic potential. Overall, recombinant ALOX12 serves as a vital tool for unraveling lipid-mediated signaling networks and advancing precision medicine approaches.
×
