| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALOX15 |
| Uniprot No | P16050 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-662aa |
| 氨基酸序列 | GLYRIRVST GASLYAGSNN QVQLWLVGQH GEAALGKRLW PARGKETELK VEVPEYLGPL LFVKLRKRHL LKDDAWFCNW ISVQGPGAGD EVRFPCYRWV EGNGVLSLPE GTGRTVGEDP QGLFQKHREE ELEERRKLYR WGNWKDGLIL NMAGAKLYDL PVDERFLEDK RVDFEVSLAK GLADLAIKDS LNVLTCWKDL DDFNRIFWCG QSKLAERVRD SWKEDALFGY QFLNGANPVV LRRSAHLPAR LVFPPGMEEL QAQLEKELEG GTLFEADFSL LDGIKANVIL CSQQHLAAPL VMLKLQPDGK LLPMVIQLQL PRTGSPPPPL FLPTDPPMAW LLAKCWVRSS DFQLHELQSH LLRGHLMAEV IVVATMRCLP SIHPIFKLII PHLRYTLEIN VRARTGLVSD MGIFDQIMST GGGGHVQLLK QAGAFLTYSS FCPPDDLADR GLLGVKSSFY AQDALRLWEI IYRYVEGIVS LHYKTDVAVK DDPELQTWCR EITEIGLQGA QDRGFPVSLQ ARDQVCHFVT MCIFTCTGQH ASVHLGQLDW YSWVPNAPCT MRLPPPTTKD ATLETVMATL PNFHQASLQM SITWQLGRRQ PVMVAVGQHE EEYFSGPEPK AVLKKFREEL AALDKEIEIR NAKLDMPYEY LRPSVVENSV AI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALOX15重组蛋白的3篇参考文献的简要信息(模拟示例，非真实文献)：
1. **"Expression and Purification of Recombinant Human ALOX15 in Escherichia coli"**
- **作者**: Brash AR, et al.
- **摘要**: 报道了在大肠杆菌中高效表达人源ALOX15重组蛋白的方法，通过优化诱导条件及亲和层析纯化，获得具有体外催化花生四烯酸生成脂质过氧化物的活性酶。
2. **"Crystal Structure of ALOX15 Reveals Insights into Substrate Specificity"**
- **作者**: Kuhn H, et al.
- **摘要**: 解析了重组ALOX15的晶体结构，阐明了其与花生四烯酸结合的关键氨基酸残基，解释了该酶对特定磷脂底物的选择性催化机制。
3. **"Functional Characterization of Recombinant ALOX15 in Macrophage Inflammation Models"**
- **作者**: Holman TR, et al.
- **摘要**: 利用重组ALOX15研究其在巨噬细胞炎症反应中的作用，发现其通过生成脂氧素调节促炎与抗炎介质的平衡，为治疗炎症疾病提供依据。
如需真实文献，建议通过PubMed或Web of Science检索关键词“ALOX15 recombinant protein”。
**Background of ALOX15 Recombinant Protein**
ALOX15 (Arachidonate 15-lipoxygenase), also known as 15-LOX-1. is a lipid-metabolizing enzyme that catalyzes the oxidation of polyunsaturated fatty acids, such as arachidonic acid, to generate bioactive lipid mediators. These mediators, including 15-hydroxyeicosatetraenoic acid (15-HETE) and specialized pro-resolving mediators (SPMs), play critical roles in regulating inflammation, cell differentiation, and apoptosis. ALOX15 is notably involved in both pro-inflammatory and anti-inflammatory pathways, depending on cellular context and substrate availability. Its dual role in promoting or resolving inflammation has made it a focal point in studying diseases like asthma, atherosclerosis, cancer, and neurodegenerative disorders.
Recombinant ALOX15 protein is produced through genetic engineering techniques, often using bacterial, insect, or mammalian expression systems. This engineered protein retains the enzymatic activity of native ALOX15. enabling researchers to study its structure-function relationships, substrate specificity, and interactions with inhibitors or modulators in vitro. The recombinant form is essential for high-throughput drug screening, structural biology (e.g., X-ray crystallography), and mechanistic studies to elucidate its role in disease pathways.
Recent studies highlight ALOX15's involvement in ferroptosis, an iron-dependent form of cell death linked to cancer and neurodegeneration. Its ability to oxidize membrane phospholipids during ferroptosis underscores its therapeutic potential as a target for cancer treatment or neuroprotection. However, its complex biological effects necessitate precise tools like recombinant proteins to dissect its functions.
Overall, ALOX15 recombinant protein serves as a vital reagent for advancing research into lipid metabolism, inflammatory diseases, and cell death mechanisms, offering insights for developing targeted therapies.
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