| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF55 |
| Uniprot No | P22681 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-906aa |
| 氨基酸序列 | MAGNVKKSSGAGGGSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGT VDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILS RYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRN LTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIV PWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQP WSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRL GQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLT GLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLM CTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGA PSPNYDDDDDERADDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRL DLLPQRVCVPSSASALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDR PYSVGAESRPQRRPLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPV SAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMN SSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEY MTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTF GEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSF GWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASA ATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISS PAHVAT |
| 预测分子量 | 126 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF55(或相关别名UHRF2)重组蛋白研究的模拟参考文献示例。由于RNF55的研究相对较少，部分内容基于类似主题文献的推测，建议通过学术数据库进一步验证：
---
1. **文献名称**：*Recombinant RNF55 Expression and Its Role in Ubiquitination Pathways*
**作者**：Zhang Y, Liu Q, Chen W
**摘要**：本研究成功在大肠杆菌中表达了重组RNF55蛋白，并证实其通过RING结构域介导泛素化反应，调控靶蛋白降解，为癌症治疗提供潜在靶点。
2. **文献名称**：*Functional Characterization of UHRF2 (RNF55) in DNA Methylation Maintenance*
**作者**：Wang X, Tanaka K, Zhou D
**摘要**：通过体外重组UHRF2蛋白实验，揭示了其与DNA甲基转移酶的协同作用，维持表观遗传稳定性，并探讨了其在胚胎发育中的功能。
3. **文献名称**：*RNF55 Recombinant Protein Modulates Cell Cycle Progression in Glioblastoma*
**作者**：Li H, Patel R, Gomez-Manzano C
**摘要**：利用哺乳动物细胞表达的重组RNF55蛋白，研究发现其过表达可阻滞胶质母细胞瘤细胞周期于G1期，提示其抑癌潜力。
4. **文献名称**：*Structural Insights into RNF55 Ubiquitin Ligase Activity*
**作者**：Müller S, Dharmarajan V, Heideker J
**摘要**：通过晶体学解析重组RNF55的结构，阐明其底物识别机制及与E2泛素结合酶的特异性互作模式。
---
**注意**：以上为模拟文献，实际研究需查阅PubMed、Web of Science等平台(关键词：RNF55/UHRF2. recombinant protein)。若需具体文献协助，请提供更多背景信息。
RNF55. also known as RING finger protein 55 or SAG (Sensitive to Apoptosis Gene), is a member of the RING finger family of E3 ubiquitin ligases. First identified in 1999 through its ability to protect cells from apoptosis induced by redox agents, RNF55 plays a critical role in regulating cellular responses to oxidative stress. Structurally, it contains a conserved RING-H2 finger domain at its C-terminus, which confers E3 ligase activity by facilitating the transfer of ubiquitin molecules to specific substrate proteins. This post-translational modification typically targets substrates for proteasomal degradation, though it can also regulate protein localization or activity.
Functionally, RNF55 is involved in multiple signaling pathways, including the NRF2-mediated antioxidant response and the KEAP1-CUL3-RBX1 ubiquitination complex. It interacts with c-Jun and other stress-responsive transcription factors, modulating their stability and activity under oxidative conditions. Studies have shown that RNF55 is particularly responsive to reactive oxygen species (ROS), acting as both a sensor and effector in redox homeostasis. Its dual role in promoting survival (by degrading pro-apoptotic proteins) and regulating antioxidant defenses makes it a key player in cellular stress adaptation.
In disease contexts, RNF55 has been implicated in cancer progression, neurodegenerative disorders, and ischemic conditions. Overexpression has been observed in various cancers, where it may confer resistance to chemotherapy by enhancing antioxidant capacity and inhibiting apoptosis. Conversely, reduced RNF55 activity has been linked to neuronal damage in conditions like Parkinson's disease, highlighting its tissue-specific functions. Current research explores its potential as a therapeutic target, particularly in combination therapies aiming to overcome treatment resistance in malignancies. As a recombinant protein, engineered RNF55 is used in biochemical studies to investigate ubiquitination mechanisms and screen for small-molecule modulators of E3 ligase activity.
×
