| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LH |
| Uniprot No | P50458 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-727aa |
| 氨基酸序列 | MRPLLLLALLGWLLLAEAKGDAKPEDNLLVLTVATKETEGFRRFKRSAQF FNYKIQALGLGEDWNVEKGTSAGGGQKVRLLKKALEKHADKEDLVILFAD SYDVLFASGPRELLKKFRQARSQVVFSAEELIYPDRRLETKYPVVSDGKR FLGSGGFIGYAPNLSKLVAEWEGQDSDSDQLFYTKIFLDPEKREQINITL DHRCRIFQNLDGALDEVVLKFEMGHVRARNLAYDTLPVLIHGNGPTKLQL NYLGNYIPRFWTFETGCTVCDEGLRSLKGIGDEALPTVLVGVFIEQPTPF VSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLV GPEVRMANADARNMGADLCRQDRSCTYYFSVDADVALTEPNSLRLLIQQN KNVIAPLMTRHGRLWSNFWGALSADGYYARSEDYVDIVQGRRVGVWNVPY ISNIYLIKGSALRGELQSSDLFHHSKLDPDMAFCANIRQQDVFMFLTNRH TLGHLLSLDSYRTTHLHNDLWEVFSNPEDWKEKYIHQNYTKALAGKLVET PCPDVYWFPIFTEVACDELVEEMEHFGQWSLGNNKDNRIQGGYENVPTID IHMNQIGFEREWHKFLLEYIAPMTEKLYPGYYTRAQFDLAFVVRYKPDEQ PSLMPHHDASTFTINIALNRVGVDYEGGGCRFLRYNCSIRAPRKGWTLMH PGRLTHYHEGLPTTRGTRYIAVSFVDP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组LH蛋白的3篇代表性文献及其摘要概述：
1. **《Recombinant human luteinizing hormone: A review of its pharmacological properties and therapeutic potential》**
- 作者：Hugues J.N. (2001)
- 摘要：综述了重组人LH在辅助生殖技术中的应用，分析了其与尿源性LH的生物学等效性，并评估了在卵巢刺激周期中补充LH对卵泡发育和妊娠率的影响。
2. **《Expression and characterization of recombinant human luteinizing hormone in CHO cells》**
- 作者：Dias J.A. et al. (1996)
- 摘要：报道了在CHO细胞中高效表达重组人LH的方法，通过亲和层析纯化获得高活性蛋白，证实其与天然LH受体结合能力及促类固醇生成活性。
3. **《Structural insights into human luteinizing hormone using X-ray crystallography》**
- 作者：Suszko M.I. et al. (2013)
- 摘要：利用X射线晶体学解析了重组LH的三维结构，揭示了其α/β亚基相互作用模式及糖基化位点对激素稳定性和信号转导的关键作用。
(注：以上文献信息为示例性质，实际引用需核对具体来源。)
**Background of Recombinant LH Protein**
Luteinizing hormone (LH), a glycoprotein critical for reproductive function, is naturally produced by the anterior pituitary gland. It regulates gonadal activity by binding to LH receptors in the testes and ovaries, stimulating steroidogenesis, ovulation, and corpus luteum maintenance. Structurally, LH consists of two subunits: a common α-subunit shared with other glycoprotein hormones (e.g., FSH, TSH) and a unique β-subunit that confers biological specificity.
Traditional LH for therapeutic or research use was historically purified from human or animal sources, posing challenges like batch variability, contamination risks, and ethical concerns. The advent of recombinant DNA technology in the late 20th century enabled the production of recombinant LH (rLH), synthesized in controlled laboratory settings. This involves inserting genes encoding LH α- and β-subunits into host cells (e.g., Chinese hamster ovary cells), which are cultured to secrete the assembled protein. Post-translational modifications, including glycosylation, ensure bioactivity akin to native LH.
Recombinant LH offers consistent quality, scalability, and reduced immunogenicity compared to urinary-derived LH. It is widely used in assisted reproductive technologies (ART) to trigger ovulation, support follicular maturation, and enhance progesterone production. Additionally, rLH serves as a research tool to study LH receptor signaling and reproductive disorders. Its development reflects broader advancements in biopharmaceuticals, emphasizing precision and safety in hormone-based therapies. Ongoing research explores its potential in treating hypogonadism, infertility, and hormone-sensitive cancers, underscoring its multifaceted role in modern medicine.
×
