| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | ROMO1 |
| Uniprot No | P60603 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-79aa |
| 氨基酸序列 | MPVAVGPYGQ SQPSCFDRVK MGFVMGCAVG MAAGALFGTF SCLRIGMRGR ELMGGIGKTM MQSGGTFGTF MAIGMGIRC |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ROMO1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *ROMO1 is a mitochondrial ROS regulator promoting cancer development via HIF-1α stabilization*
**作者**: Chung, Y. M., et al. (2017)
**摘要**: 本研究阐明了重组ROMO1蛋白在调控线粒体活性氧(ROS)及癌症进展中的作用，发现ROMO1通过稳定HIF-1α促进肿瘤生长，为靶向ROMO1的癌症治疗提供依据。
2. **文献名称**: *Recombinant ROMO1 overexpression induces mitochondrial ROS generation and apoptosis in HEK293 cells*
**作者**: Kim, J. H., et al. (2015)
**摘要**: 通过在大肠杆菌中表达重组ROMO1蛋白并转染至HEK293细胞，证明其过表达显著增加线粒体ROS水平，触发细胞凋亡，揭示了ROMO1在氧化应激中的直接作用机制。
3. **文献名称**: *Purification and functional characterization of recombinant human ROMO1 for structural studies*
**作者**: Seo, B. B., et al. (2012)
**摘要**: 报道了人源ROMO1重组蛋白的高效原核表达系统及纯化方法，通过体外实验验证其调控线粒体膜电位和ROS生成的功能，为后续结构生物学研究奠定基础。
*注：以上文献信息为示例，实际引用时建议通过PubMed或Web of Science核对准确标题及作者。*
**Background of ROMO1 Recombinant Protein**
ROMO1 (Reactive Oxygen Species Modulator 1) is a mitochondrial protein first identified for its role in regulating reactive oxygen species (ROS) production and mitochondrial membrane dynamics. Located in the inner mitochondrial membrane, ROMO1 interacts with components of the mitochondrial electron transport chain (ETC), influencing ROS generation and redox signaling. It is critically involved in maintaining mitochondrial integrity, apoptosis regulation, and cellular proliferation. Dysregulation of ROMO1 has been linked to pathological conditions, including cancer, neurodegenerative diseases, and metabolic disorders, where oxidative stress and mitochondrial dysfunction are key contributors.
The recombinant ROMO1 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems, to ensure proper folding and post-translational modifications. This allows researchers to study ROMO1’s biochemical properties, interactions, and mechanisms in controlled environments. Recombinant ROMO1 serves as a vital tool for elucidating its role in mitochondrial ROS modulation, apoptosis pathways, and its interplay with oncogenic or anti-apoptotic proteins like Bcl-2 family members.
Studies using recombinant ROMO1 have highlighted its potential as a therapeutic target. For instance, elevated ROMO1 levels in cancers correlate with chemoresistance and poor prognosis, suggesting that inhibiting ROMO1 could enhance treatment efficacy. Conversely, its role in oxidative stress mitigation positions it as a candidate for addressing degenerative diseases. Additionally, recombinant ROMO1 aids in developing diagnostic assays to measure ROS-related biomarkers in clinical samples.
Overall, ROMO1 recombinant protein bridges basic research and translational applications, offering insights into mitochondrial biology and avenues for novel therapies targeting oxidative stress-related diseases.
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