| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFATC1 |
| Uniprot No | O95644 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-943aa |
| 氨基酸序列 | MPSTSFPVPSKFPLGPAAAVFGRGETLGPAPRAGGTMKSAEEEHYGYASSNVSPALPLPTAHSTLPAPCHNLQTSTPGIIPPADHPSGYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGFPRGLGACTLLGSPRHSPSTSPRASVTEESWLGARSSRPASPCNKRKYSLNGRQPPYSPHHSPTPSPHGSPRVSVTDDSWLGNTTQYTSSAIVAAINALTTDSSLDLGDGVPVKSRKTTLEQPPSVALKVEPVGEDLGSPPPPADFAPEDYSSFQHIRKGGFCDQYLAVPQHPYQWAKPKPLSPTSYMSPTLPALDWQLPSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPIVQLHGYLENEPLMLQLFIGTADDRLLRPHAFYQVHRITGKTVSTTSHEAILSNTKVLEIPLLPENSMRAVIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQPSGRTLSLQVASNPIECSQRSAQELPLVEKQSTDSYPVVGGKKMVLSGHNFLQDSKVIFVEKAPDGHHVWEMEAKTDRDLCKPNSLVVEIPPFRNQRITSPVHVSFYVCNGKRKRSQYQRFTYLPANVPIIKTEPTDDYEPAPTCGPVSQGLSPLPRPYYSQQLAMPPDPSSCLVAGFPPCPQRSTLMPAAPGVSPKLHDLSPAAYTKGVASPGHCHLGLPQPAGEAPAVQDVPRPVATHPGSPGQPPPALLPQQVSAPPSSSCPPGLEHSLCPSSPSPPLPPATQEPTCLQPCSPACPPATGRPQHLPSTVRRDESPTAGPRLLPEVHEDGSPNLAPIPVTVKREPEELDQLYLDDVNEIIRNDLSSTSTHS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NFATC1重组蛋白的3篇模拟参考文献，供参考：
1. **《NFATC1重组蛋白的体外表达及功能分析》**
- 作者：Hogan, P.G. 等
- 摘要：研究通过大肠杆菌系统重组表达NFATC1蛋白，验证其DNA结合活性，并证明其磷酸化修饰对核转位调控的关键作用。
2. **《NFAT信号通路中重组NFATC1与钙调神经磷酸酶的相互作用》**
- 作者：Rao, A.; Luo, C.
- 摘要：利用重组NFATC1蛋白研究其与钙调神经磷酸酶的结合机制，揭示免疫应答中NFAT去磷酸化激活的分子基础。
3. **《NFATC1重组蛋白的结构解析及其转录调控功能》**
- 作者：Graef, I.A.; Crabtree, G.R.
- 摘要：通过X射线晶体学解析NFATC1重组蛋白结构，阐明其与DNA及协同转录因子的结合模式，提出其在T细胞分化中的动态调控模型。
4. **《靶向NFATC1重组蛋白的抗肿瘤药物筛选研究》**
- 作者：Wu, H.; Li, X.
- 摘要：基于NFATC1重组蛋白构建高通量筛选平台，发现小分子抑制剂可阻断其与DNA结合，为癌症治疗提供潜在策略。
(注：以上文献及作者为模拟生成，实际研究请参考真实数据库如PubMed。)
Nuclear factor of activated T cells 1 (NFATC1), also known as NFAT2. is a transcription factor belonging to the NFAT protein family, which plays critical roles in immune response, cell differentiation, and development. It is characterized by a conserved N-terminal regulatory domain that interacts with calcium-sensitive phosphatase calcineurin and a C-terminal DNA-binding domain. In resting cells, NFATC1 resides in the cytoplasm in a phosphorylated state. Upon calcium signaling, calcineurin dephosphorylates NFATC1. triggering its nuclear translocation to regulate target gene expression. NFATC1 is particularly vital in T-cell activation, osteoclastogenesis, and cardiovascular development, with dysregulation linked to autoimmune diseases, cancer, and bone disorders.
Recombinant NFATC1 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research and therapeutic applications. This engineered protein retains key functional domains, enabling studies on its DNA-binding activity, protein interactions, and phosphorylation dynamics. Researchers utilize recombinant NFATC1 to dissect signaling pathways, screen drug candidates targeting calcineurin-NFAT interactions, and model pathological conditions caused by NFAT dysregulation. Its isoforms, generated by alternative splicing, add complexity to its functional diversity, necessitating isoform-specific studies. Recombinant variants may include tagged versions (e.g., GFP, His-tag) for tracking or purification. As NFAT pathways are implicated in immunosuppression and tumor microenvironments, recombinant NFATC1 serves as a tool to explore therapeutic strategies, including modulating immune responses or targeting oncogenic signaling. Its role in transcriptional networks also supports studies in developmental biology and regenerative medicine.
×
