| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFATC2 |
| Uniprot No | Q13469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-925aa |
| 氨基酸序列 | MNAPERQPQPDGGDAPGHEPGGSPQDELDFSILFDYEYLNPNEEEPNAHKVASPPSGPAYPDDVLDYGLKPYSPLASLSGEPPGRFGEPDRVGPQKFLSAAKPAGASGLSPRIEITPSHELIQAVGPLRMRDAGLLVEQPPLAGVAASPRFTLPVPGFEGYREPLCLSPASSGSSASFISDTFSPYTSPCVSPNNGGPDDLCPQFQNIPAHYSPRTSPIMSPRTSLAEDSCLGRHSPVPRPASRSSSPGAKRRHSCAEALVALPPGASPQRSRSPSPQPSSHVAPQDHGSPAGYPPVAGSAVIMDALNSLATDSPCGIPPKMWKTSPDPSPVSAAPSKAGLPRHIYPAVEFLGPCEQGERRNSAPESILLVPPTWPKPLVPAIPICSIPVTASLPPLEWPLSSQSGSYELRIEVQPKPHHRAHYETEGSRGAVKAPTGGHPVVQLHGYMENKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGNTKVLEIPLEPKNNMRATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHIPESSGRIVSLQTASNPIECSQRSAHELPMVERQDTDSCLVYGGQQMILTGQNFTSESKVVFTEKTTDGQQIWEMEATVDKDKSQPNMLFVEIPEYRNKHIRTPVKVNFYVINGKRKRSQPQHFTYHPVPAIKTEPTDEYDPTLICSPTHGGLGSQPYYPQHPMVAESPSCLVATMAPCQQFRTGLSSPDARYQQQNPAAVLYQRSKSLSPSLLGYQQPALMAAPLSLADAHRSVLVHAGSQGQSSALLHPSPTNQQASPVIHYSPTNQQLRCGSHQEFQHIMYCENFAPGTTRPGPPPVSQGQRLSPGSYPTVIQQQNATSQRAAKNGPPVSDQKEVLPAGVTIKQEQNLDQTYLDDVNEIIRKEFSGPPARNQT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NFATC2重组蛋白的3篇参考文献示例(文献信息为模拟生成，仅供参考)：
1. **文献名称**: "Recombinant NFATC2 Protein Enhances T-cell Activation in Autoimmune Disease Models"
**作者**: Li, X. et al.
**摘要**: 该研究通过原核表达系统制备了重组NFATC2蛋白，并验证其促进T细胞活化的功能。实验表明，该蛋白在体外可增强IL-2表达，提示其在自身免疫疾病中的调控作用。
2. **文献名称**: "Structural Analysis of NFATC2-DNA Complex Using Purified Recombinant Protein"
**作者**: Kumar, R. & Garcia, K.C.
**摘要**: 利用昆虫细胞表达系统纯化NFATC2重组蛋白，通过X射线晶体学解析其与DNA结合的结构域三维结构，揭示了关键氨基酸残基在调控基因转录中的作用机制。
3. **文献名称**: "NFATC2 Recombinant Protein Modulates Osteoclast Differentiation in vitro"
**作者**: Tanaka, S. et al.
**摘要**: 研究通过哺乳动物细胞表达体系获得功能性NFATC2重组蛋白，证实其通过RANKL信号通路调控破骨细胞分化，为骨质疏松治疗提供潜在靶点。
注：以上文献为示例，实际引用时需通过PubMed/Web of Science等平台检索真实文献。建议使用关键词"NFATC2 recombinant protein"+"expression/function/structure"进行精准筛选。
NFATC2 (Nuclear Factor of Activated T-Cells Cytoplasmic 2) is a transcription factor belonging to the NFAT family, which plays pivotal roles in immune response, cell differentiation, and development. Initially identified in T lymphocytes, NFATC2 regulates gene expression by translocating to the nucleus upon dephosphorylation by calcineurin, a calcium-dependent phosphatase. This activation mechanism links extracellular signals, such as antigen recognition or cytokine stimulation, to transcriptional programs governing immune cell activation, cytokine production, and apoptosis. Beyond immunology, NFATC2 is involved in osteoclastogenesis, angiogenesis, and cardiac development, highlighting its broad functional relevance.
Recombinant NFATC2 protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and regulatory mechanisms. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant protein retains key functional domains, including the N-terminal regulatory domain (with phosphorylation sites controlling nuclear localization) and the C-terminal DNA-binding domain. Purification methods often employ affinity tags (e.g., His-tag) for high yield and purity.
Structurally, NFATC2 binds cooperatively with partner proteins like AP-1 to target DNA motifs in promoters of genes such as IL-2. TNF-α, and CD40L. Dysregulation of NFATC2 is implicated in autoimmune diseases, cancer, and transplant rejection, making it a therapeutic target. Recombinant NFATC2 enables studies on its post-translational modifications, DNA-binding kinetics, and inhibitor screening. Additionally, it serves as a tool to explore cross-talk with signaling pathways like NF-κB and MAPK. By providing a controllable source of the protein, recombinant NFATC2 accelerates mechanistic research and drug discovery, bridging molecular insights to potential clinical applications.
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