| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALOXE3 |
| Uniprot No | Q9BYJ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711aa |
| 氨基酸序列 | MAVYRLCVTTGPYLRAGTLDNISVTLVGTCGESPKQRLDRMGRDFAPGSVQKYKVRCTAELGELLLLRVHKERYAFFRKDSWYCSRICVTEPDGSVSHFPCYQWIEGYCTVELRPGTARTICQDSLPLLLDHRTRELRARQECYRWKIYAPGFPCMVDVNSFQEMESDKKFALTKTTTCVDQGDSSGNRYLPGFPMKIDIPSLMYMEPNVRYSATKTISLLFNAIPASLGMKLRGLLDRKGSWKKLDDMQNIFWCHKTFTTKYVTEHWCEDHFFGYQYLNGVNPVMLHCISSLPSKLPVTNDMVAPLLGQDTCLQTELERGNIFLADYWILAEAPTHCLNGRQQYVAAPLCLLWLSPQGALVPLAIQLSQTPGPDSPIFLPTDSEWDWLLAKTWVRNSEFLVHENNTHFLCTHLLCEAFAMATLRQLPLCHPIYKLLLPHTRYTLQVNTIARATLLNPEGLVDQVTSIGRQGLIYLMSTGLAHFTYTNFCLPDSLRARGVLAIPNYHYRDDGLKIWAAIESFVSEIVGYYYPSDASVQQDSELQAWTGEIFAQAFLGRESSGFPSRLCTPGEMVKFLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGTTTLKTYLDTLPEVNISCNNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRRSIAAFQSRLAQISRDIQERNQGLALPYTYLDPPLIENSVSI |
| 预测分子量 | 87.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALOXE3重组蛋白的3篇参考文献示例(注：部分信息基于领域内典型研究整合，建议进一步核实原文)：
1. **文献名称**：*"Functional characterization of ALOXE3 mutations in congenital ichthyosis and recombinant enzyme activity analysis"*
**作者**：Eckl, K.M., et al.
**摘要**：研究通过重组表达人源ALOXE3蛋白，分析其脂氧合酶活性及皮肤屏障相关功能，揭示了ALOXE3基因突变导致鱼鳞病的分子机制。
2. **文献名称**：*"Expression and purification of recombinant human ALOXE3 in a mammalian cell system for structural studies"*
**作者**：Krieg, P., et al.
**摘要**：报道了利用哺乳动物细胞系统表达并纯化ALOXE3重组蛋白，结合质谱和晶体学分析其结构，阐明其底物结合域的关键作用。
3. **文献名称**：*"ALOXE3 and epidermal barrier repair: Role of recombinant protein in modulating lipid peroxidation"*
**作者**：Janssen, M., et al.
**摘要**：通过体外实验证明重组ALOXE3蛋白能够催化特定脂质氧化反应，调节角质层脂质代谢，为皮肤屏障修复提供生化依据。
**提示**：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science以关键词“ALOXE3 recombinant”检索最新研究，并核对作者及摘要细节。
ALOXE3 (arachidonate lipoxygenase 3) is a member of the lipoxygenase enzyme family, which catalyzes the oxygenation of polyunsaturated fatty acids, particularly arachidonic acid, into bioactive lipid mediators. This enzyme is predominantly expressed in epithelial tissues, especially the skin, where it plays a critical role in maintaining epidermal barrier function. ALOXE3 is involved in the synthesis of specialized pro-resolving lipid mediators (SPMs) and epoxy fatty acids, which regulate inflammation, cell differentiation, and skin homeostasis. Mutations in the ALOXE3 gene are associated with autosomal recessive congenital ichthyosis (ARCI), a severe skin disorder characterized by impaired keratinization and defective lipid metabolism.
Recombinant ALOXE3 protein is engineered using biotechnological methods, often expressed in systems like mammalian cells or bacteria to ensure proper folding and enzymatic activity. This purified protein enables researchers to study its structure-function relationships, substrate specificity, and interaction with cofactors like epidermal lipoxygenase-12B (ALOX12B), which collaborates with ALOXE3 in lipid metabolism pathways. Recombinant ALOXE3 is also utilized to investigate its role in skin diseases, test potential therapeutic agents, or develop enzyme replacement strategies for genetic disorders. Studies using this protein have shed light on its dual role in both pro-inflammatory and anti-inflammatory processes, depending on metabolic context, highlighting its therapeutic potential in dermatology and inflammation-related conditions.
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