| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MC5R |
| Uniprot No | P33032 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-325aa |
| 氨基酸序列 | MNSSFHLHFLDLNLNATEGNLSGPNVKNKSSPCEDMGIAVEVFLTLGVISLLENILVIGAIVKNKNLHSPMYFFVCSLAVADMLVSMSSAWETITIYLLNNKHLVIADAFVRHIDNVFDSMICISVVASMCSLLAIAVDRYVTIFYALRYHHIMTARRSGAIIAGIWAFCTGCGIVFILYSESTYVILCLISMFFAMLFLLVSLYIHMFLLARTHVKRIAALPGASSARQRTSMQGAVTVTMLLGVFTVCWAPFFLHLTLMLSCPQNLYCSRFMSHFNMYLILIMCNSVMDPLIYAFRSQEMRKTFKEIICCRGFRIACSFPRRD |
| 预测分子量 | 38.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MC5R重组蛋白的3篇参考文献概览：
1. **文献名称**：*Expression and functional characterization of melanocortin receptor 5 (MC5R) in mammalian cells*
**作者**：Smith A, et al.
**摘要**：研究报道了在HEK293细胞中重组表达人源MC5R，通过cAMP信号通路验证其功能活性，证实MC5R能被α-MSH激活，并探讨了其在脂代谢中的潜在作用。
2. **文献名称**：*Purification and ligand-binding analysis of recombinant MC5R from insect cells*
**作者**：Chen L, et al.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达MC5R重组蛋白，经亲和层析纯化后，采用放射性配体结合实验测定其对不同黑皮质素肽的亲和力，揭示了MC5R的选择性结合特性。
3. **文献名称**：*Structural insights into MC5R activation through cryo-EM analysis*
**作者**：Wang Y, et al.
**摘要**：通过冷冻电镜解析MC5R重组蛋白与激动剂复合物的三维结构，阐明了受体激活的分子机制，为靶向MC5R的药物设计提供了结构基础。
(注：上述文献为示例，实际引用需根据具体论文调整。)
Melanocortin 5 receptor (MC5R) is a member of the melanocortin receptor family, a group of G protein-coupled receptors (GPCRs) that bind melanocortin peptides. Primarily expressed in exocrine glands, skin, immune cells, and adipose tissue, MC5R plays critical roles in lipid metabolism, sebum production, thermoregulation, and immune modulation. Unlike other melanocortin receptors (e.g., MC1R in pigmentation), MC5R’s involvement in sebaceous gland regulation has drawn attention for its potential link to skin disorders, metabolic syndromes, and inflammatory conditions. Activation by α-MSH or synthetic ligands triggers cAMP signaling, influencing cellular processes like lipid secretion and anti-inflammatory responses.
Recombinant MC5R protein, engineered via heterologous expression systems (e.g., mammalian, insect, or yeast cells), enables in vitro studies to dissect its structural and functional properties. Its hydrophobic transmembrane domains pose challenges in purification, often requiring detergent solubilization and affinity tagging. Researchers utilize recombinant MC5R to screen therapeutic ligands, map binding interfaces, and study receptor dimerization or downstream signaling crosstalk. Emerging evidence suggests MC5R as a target for treating acne (via sebum modulation), obesity (through lipid metabolism), and autoimmune diseases (by immune cell regulation). However, its precise physiological roles remain partially elusive, necessitating further studies using recombinant tools to clarify tissue-specific functions and therapeutic potential. Advances in cryo-EM and computational modeling are expected to accelerate MC5R-targeted drug discovery.
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