| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AIM1 |
| Uniprot No | Q9Y4K1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AIM1重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**：*"Structural characterization of recombinant human AIM1 reveals a novel βγ-crystallin fold"*
**作者**：Smith et al.
**摘要**：本研究通过重组表达人源AIM1蛋白，解析其晶体结构，发现其具有独特的βγ-晶状体蛋白结构域，可能参与细胞骨架调控和肿瘤抑制功能。
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2. **文献名称**：*"Functional analysis of AIM1 in melanoma progression using recombinant protein-based assays"*
**作者**：Johnson & Lee
**摘要**：作者通过大肠杆菌表达重组AIM1蛋白，验证其与肌动蛋白的结合能力，并发现AIM1通过调控细胞迁移抑制黑色素瘤转移。
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3. **文献名称**：*"Recombinant AIM1 protein suppresses hepatocellular carcinoma growth via Wnt/β-catenin signaling inhibition"*
**作者**：Wang et al.
**摘要**：该研究利用哺乳动物细胞系统表达重组AIM1.证明其可通过阻断Wnt通路抑制肝癌细胞增殖，为靶向治疗提供潜在策略。
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注：以上文献信息为示例，实际引用需根据具体研究内容检索PubMed或Google Scholar获取原文。
AIM1 (Absent in Melanoma 1) is a protein initially identified for its reduced expression in melanoma cells, suggesting a potential role in tumor suppression. It belongs to the βγ-crystallin superfamily and is structurally characterized by two conserved βγ-crystallin domains, which are typically associated with calcium-binding properties. AIM1 is also known as CRYBG1 (Crystallin Beta-Gamma Domain-Containing 1) and plays critical roles in cellular processes such as cytoskeletal organization, cell adhesion, and apoptosis. Its involvement in maintaining epithelial tissue integrity and regulating cell migration has linked it to cancer progression, particularly in epithelial-mesenchymal transition (EMT) and metastasis.
Recombinant AIM1 protein is produced using biotechnological systems like *E. coli* or mammalian cell cultures, enabling large-scale purification for research and therapeutic applications. The recombinant form retains functional domains necessary for studying its interactions with cytoskeletal components (e.g., actin filaments) and signaling pathways (e.g., Wnt/β-catenin). Researchers utilize AIM1 recombinant protein to investigate its tumor-suppressive mechanisms, including inhibition of invasive cell behavior and modulation of apoptosis in cancer models. Additionally, its role in eye lens development, attributed to crystallin-like structural properties, has spurred interest in ophthalmology and developmental biology.
Beyond cancer, AIM1 is implicated in inflammatory diseases and tissue homeostasis, with studies exploring its extracellular matrix interactions. The protein’s dual role in both intracellular structural regulation and extracellular signaling highlights its versatility. Recombinant AIM1 serves as a vital tool for drug discovery, antibody development, and mechanistic studies aiming to harness its therapeutic potential in oncology and regenerative medicine. Its conserved structure across species further supports translational research, bridging molecular insights to clinical applications.
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