| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNS3 |
| Uniprot No | Q68CZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNS3重组蛋白的模拟参考文献示例(请注意，这些文献为虚构示例，仅供格式参考)：
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1. **文献名称**: *"Recombinant TNS3 Protein Expression and Its Role in Cell Adhesion"*
**作者**: Smith J, et al.
**摘要**: 本研究利用哺乳动物表达系统成功表达并纯化了TNS3重组蛋白，验证其在调节细胞黏附和迁移中的功能。实验表明，TNS3通过整合素信号通路增强细胞与细胞外基质的相互作用。
2. **文献名称**: *"Structural Characterization of Recombinant TNS3 and Its Interaction with PI3K"*
**作者**: Lee H, Zhang Y.
**摘要**: 通过X射线晶体学解析了TNS3重组蛋白的SH2结构域三维结构，揭示了其与PI3K激酶特异性结合的分子机制，为靶向TNS3的癌症治疗提供了结构基础。
3. **文献名称**: *"Development of a Bacterial Expression System for High-Yield TNS3 Production"*
**作者**: Patel R, et al.
**摘要**: 优化了大肠杆菌中TNS3重组蛋白的可溶性表达条件，获得高纯度蛋白用于抗体开发，并验证其在免疫印迹和ELISA中的适用性。
4. **文献名称**: *"TNS3 Recombinant Protein Suppresses Metastasis in Colorectal Cancer Models"*
**作者**: Garcia M, et al.
**摘要**: 体外和体内实验表明，外源性TNS3重组蛋白通过抑制Wnt/β-catenin信号通路，显著降低结直肠癌细胞的侵袭和转移能力。
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**提示**：如需真实文献，建议通过PubMed或Google Scholar搜索关键词“TNS3 recombinant”、“Tensin 3 expression”等获取最新研究。
**Background of TNS3 Recombinant Protein**
TNS3 (Tensin 3) is a multidomain protein belonging to the tensin family, which includes critical regulators of cell adhesion, migration, and signaling. Structurally, TNS3 contains an N-terminal region with a phosphotyrosine-binding (PTB) domain, a central actin-binding domain, and a C-terminal Src homology 2 (SH2) domain, enabling interactions with cytoskeletal components and signaling molecules. It localizes to focal adhesion sites, where it bridges integrin-mediated extracellular matrix (ECM) signaling to intracellular actin dynamics, playing a role in maintaining cell shape, motility, and mechanotransduction.
Recombinant TNS3 protein is engineered *in vitro* using expression systems (e.g., bacterial, mammalian, or insect cells) to produce purified, functional protein for research. Its production often involves tagging (e.g., His-tag) for simplified purification and detection. Studies highlight TNS3's involvement in pathways like PI3K/AKT and Rho GTPase signaling, influencing cell proliferation, survival, and metastasis. Dysregulation of TNS3 is linked to pathological conditions, including cancer progression, fibrosis, and cardiovascular diseases, where altered ECM interactions contribute to disease mechanisms.
Research applications of recombinant TNS3 include *in vitro* binding assays to map protein-protein interactions (e.g., integrins, tyrosine kinases), functional studies in cell migration/invasion models, and screening for therapeutic agents targeting adhesion-related pathologies. Its role in tumor suppression or promotion remains context-dependent, with tissue-specific expression patterns and splice variants adding complexity. For instance, TNS3 may act as a metastasis suppressor in certain cancers but promote invasiveness in others, underscoring its dual regulatory potential.
Overall, TNS3 recombinant protein serves as a vital tool for dissecting focal adhesion biology and developing strategies to modulate cell-ECM communication in disease. Ongoing research aims to clarify its isoform-specific functions and therapeutic relevance.
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