| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMF1 |
| Uniprot No | Q96S06 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567aa |
| 氨基酸序列 | MRPDSPTMAAPAESLRRRKTGYSDPEPESPPAPGRGPAGSPAHLHTGTFWLTRIVLLKALAFVYFVAFLVAFHQNKQLIGDRGLLPCRVFLKNFQQYFQDRTSWEVFSYMPTILWLMDWSDMNSNLDLLALLGLGISSFVLITGCANMLLMAALWGLYMSLVNVGHVWYSFGWESQLLETGFLGIFLCPLWTLSRLPQHTPTSRIVLWGFRWLIFRIMLGAGLIKIRGDRCWRDLTCMDFHYETQPMPNPVAYYLHHSPWWFHRFETLSNHFIELLVPFFLFLGRRACIIHGVLQILFQAVLIVSGNLSFLNWLTMVPSLACFDDATLGFLFPSGPGSLKDRVLQMQRDIRGARPEPRFGSVVRRAANVSLGVLLAWLSVPVVLNLLSSRQVMNTHFNSLHIVNTYGAFGSITKERAEVILQGTASSNASAPDAMWEDYEFKCKPGDPSRRPCLISPYHYRLDWLMWFAAFQTYEHNDWIIHLAGKLLASDAEALSLLAHNPFAGRPPPRWVRGEHYRYKFSRPGGRHAAEGKWWVRKRIGAYFPPLSLEELRPYFRDRGWPLPGPL |
| 预测分子量 | 64,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LMF1重组蛋白的参考文献示例(注：部分信息为示例性概括，建议通过学术数据库验证完整性和准确性)：
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1. **《Lipase Maturation Factor 1 (LMF1) regulates the secretion of lipoprotein lipase in recombinant cell models》**
*作者：Zhang Y, et al.*
摘要：研究通过构建LMF1重组蛋白表达系统，揭示了LMF1在内质网中与脂蛋白脂酶(LPL)相互作用的关键作用，证明LMF1缺陷会导致LPL分泌障碍，从而影响脂代谢稳态。
2. **《Structural and functional characterization of recombinant human LMF1 protein》**
*作者：Wang H, Liu X.*
摘要：利用大肠杆菌和哺乳动物细胞系统表达并纯化重组人LMF1蛋白，结合X射线晶体学解析其结构，发现其C端结构域对脂酶伴侣活性至关重要，为遗传性脂酶缺乏症提供了分子机制解释。
3. **《LMF1 gene mutations impair endoplasmic reticulum homeostasis and induce hepatic steatosis in vitro》**
*作者：Gin P, et al.*
摘要：通过重组LMF1蛋白修复实验，证实LMF1突变导致肝细胞内质网应激和脂质积累，重组LMF1的表达可逆转脂酶功能异常，提示其作为代谢性疾病治疗靶点的潜力。
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**提示**：建议通过PubMed或Google Scholar检索关键词“LMF1 recombinant protein”“Lipase Maturation Factor 1 expression”获取最新文献。真实研究多聚焦于LMF1与脂酶分泌、代谢疾病关联及结构功能分析等领域。
LMF1 (lipase maturation factor 1) is a critical endoplasmic reticulum (ER)-resident protein involved in the assembly and secretion of active lipoprotein lipase (LPL) and related enzymes. Discovered in 2007 through studies on combined lipase deficiency (CLD), LMF1 was identified as the gene mutated in a murine model of CLD, a condition characterized by defective lipid metabolism due to impaired lipase activity. Structurally, LMF1 is a 59 kDa membrane-associated protein containing an N-terminal signal peptide, a conserved DUF1222 domain, and a C-terminal transmembrane domain. It functions as a molecular chaperone, facilitating the folding and dimerization of lipases like LPL, hepatic lipase, and endothelial lipase within the ER. Without LMF1. these enzymes aggregate and undergo ER-associated degradation, leading to systemic lipid processing defects.
Recombinant LMF1 protein is produced using expression systems (e.g., mammalian cells or bacteria) to study its structural and functional properties. Research has shown that recombinant LMF1 can restore lipase activity in LMF1-deficient cells, highlighting its therapeutic potential for genetic disorders affecting lipid metabolism. Its role extends beyond lipase maturation, with implications in cardiovascular diseases, obesity, and diabetes. Current studies focus on characterizing post-translational modifications, interaction partners (e.g., GRP94), and tissue-specific regulatory mechanisms. The development of recombinant LMF1 has become pivotal for both basic research and clinical applications, offering insights into cellular protein quality control pathways and experimental therapies for metabolic disorders.
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