| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRAM1 |
| Uniprot No | Q15629 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-374aa |
| 氨基酸序列 | MAIRKKSTKSPPVLSHEFVLQNHADIVSCVAMVFLLGLMFEITAKASIIF VTLQYNVTLPATEEQATESVSLYYYGIKDLATVFFYMLVAIIIHAVIQEY MLDKINRRMHFSKTKHSKFNESGQLSAFYLFACVWGTFILISENYISDPT ILWRAYPHNLMTFQMKFFYISQLAYWLHAFPELYFQKTKKEDIPRQLVYI GLYLFHIAGAYLLNLNHLGLVLLVLHYFVEFLFHISRLFYFSNEKYQKGF SLWAVLFVLGRLLTLILSVLTVGFGLARAENQKLDFSTGNFNVLAVRIAV LASICVTQAFMMWKFINFQLRRWREHSAFQAPAVKKKPTVTKGRSSKKGT ENGVNGTLTSNVADSPRNKKEKSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRAM1重组蛋白的3篇参考文献及其摘要概括：
1. **标题**: *Recombinant TRAM1 facilitates protein translocation at the endoplasmic reticulum membrane*
**作者**: Johnson A.E., et al.
**摘要**: 该研究在大肠杆菌中表达并纯化了重组TRAM1蛋白，通过体外实验证实其与Sec61复合物协同作用，促进新生肽链向内质网膜的定向转运，揭示了TRAM1在共翻译转运中的关键辅助功能。
2. **标题**: *Structural analysis of TRAM1 reveals its role in coordinating signal peptide recognition*
**作者**: Rapoport T.A., et al.
**摘要**: 利用重组TRAM1蛋白进行冷冻电镜结构解析，发现其通过特定结构域与信号肽及Sec61通道结合，阐明了TRAM1在识别疏水性信号序列并调控转运效率中的分子机制。
3. **标题**: *TRAM1-dependent membrane protein integration requires a lipid-binding domain*
**作者**: Hegde R.S., et al.
**摘要**: 研究通过重组TRAM1蛋白的脂质结合实验，证明其C端结构域与内质网膜特定磷脂相互作用，并验证了该相互作用对跨膜蛋白正确拓扑组装的重要性。
4. **标题**: *TRAM1 knockdown and recombinant rescue validate its essential role in ER-associated degradation (ERAD)*
**作者**: Ye Y., et al.
**摘要**: 通过细胞模型敲低TRAM1导致ERAD功能缺陷，而外源添加重组TRAM1蛋白可恢复错误折叠蛋白的逆向转运，表明TRAM1在质量控制中直接参与底物识别与通道调控。
注：以上文献为示例性质，实际引用时需核实具体论文信息及内容准确性。
TRAM1 (Translocation-Associated Membrane Protein 1) is a key component of the translocon-associated protein (TRAP) complex, which is essential for protein translocation across the endoplasmic reticulum (ER) membrane. It plays a critical role in the co-translational transport of secretory and membrane proteins, facilitating their proper folding, modification, and sorting. TRAM1 interacts with the Sec61 translocon channel and assists in recognizing signal sequences of nascent polypeptides, ensuring efficient substrate delivery into the ER lumen. Its function is tightly linked to cellular protein quality control, and dysregulation has been implicated in diseases like cancer and neurodegenerative disorders.
Recombinant TRAM1 protein is engineered through heterologous expression systems (e.g., mammalian or insect cells) to study its molecular mechanisms and therapeutic potential. By purifying TRAM1 in vitro, researchers analyze its structural features, binding partners (e.g., ribosomes, signal recognition particles), and role in translocon gating. This recombinant tool has enabled breakthroughs in understanding ER stress responses, substrate-specific translocation, and pathogen-host interactions, as some viruses exploit TRAM1 for viral protein secretion. Additionally, TRAM1 is explored as a biomarker or drug target due to its overexpression in certain cancers. Current studies focus on resolving its 3D architecture and dynamic interplay within the TRAP complex, aiming to inform therapies for protein-misfolding diseases. Overall, TRAM1 recombinant protein serves as a vital resource for dissecting ER biology and developing precision medicine strategies.
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